Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts

P. Knipscheer; M. Raschle; O.D. Scharer; J.C. Walter

doi:10.1007/978-1-61779-998-3_16

Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts

P. Knipscheer, M. Raschle, O.D. Scharer, J.C. Walter

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

29 Citations (Scopus)

Abstract

Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

Original language	English
Pages (from-to)	221-243
Journal	Methods in Molecular Biology
Volume	920
DOIs	https://doi.org/10.1007/978-1-61779-998-3_16
Publication status	Published - 2012

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title = "Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts",

abstract = "Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.",

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AU - Raschle, M.

AU - Scharer, O.D.

AU - Walter, J.C.

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N2 - Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

AB - Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

U2 - 10.1007/978-1-61779-998-3_16

DO - 10.1007/978-1-61779-998-3_16

M3 - Article

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VL - 920

SP - 221

EP - 243

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts

Abstract

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