Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome

Lucas William Mendes (Corresponding author), Miriam Gonçalves De Chaves, Mariley de Cássia da Fonseca, Rodrigo Mendes, Jos M Raaijmakers, Siu Mui Tsai

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Abstract

The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community towards a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections.
Original languageEnglish
Article number2019.02252
JournalFrontiers in Microbiology
Volume10
Early online date2019
DOIs
Publication statusPublished - 2019

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Bibliographical note

6821, ME; Data Archiving: Data archived at MG-RAST
The metatranscriptome data used in this study are available in
MG-RAST server under the project ‘Common Bean Rhizosphere
Metatranscriptome’ (mgp20659). Not NIOO data

Keywords

  • international
  • Plan_S-Compliant_OA

Cite this

Mendes, L. W., Gonçalves De Chaves, M., da Fonseca, M. D. C., Mendes, R., Raaijmakers, J. M., & Tsai, S. M. (2019). Resistance breeding of common bean shapes the physiology of the rhizosphere microbiome. Frontiers in Microbiology, 10, [2019.02252]. https://doi.org/10.3389/fmicb.2019.02252