Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

I. Geudens; R. Herpers; K. Hermans; I. Segura; C. Ruiz de Almodovar; J. Bussmann; F. De Smet; W. Vandevelde; B.M. Hogan; A. Siekmann; F. Claes; J. Moore; A.S. Pistocchi; S. Loges; M. Mazzone; G. Mariggi; F. Bruyere; F. Cotelli; D. Kerjaschki; A. Noel; J.M. Foidart; H. Gerhardt; A. Ny; T. Langenberg; N. Lawson; H.J. Duckers; S. Schulte-Merker; P. Carmeliet; M. Dewerchin

doi:10.1161/ATVBAHA.110.203034

Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

I. Geudens, R. Herpers, K. Hermans, I. Segura, C. Ruiz de Almodovar, J. Bussmann, F. De Smet, W. Vandevelde, B.M. Hogan, A. Siekmann, F. Claes, J. Moore, A.S. Pistocchi, S. Loges, M. Mazzone, G. Mariggi, F. Bruyere, F. Cotelli, D. Kerjaschki, A. NoelJ.M. Foidart, H. Gerhardt, A. Ny, T. Langenberg, N. Lawson, H.J. Duckers, S. Schulte-Merker, P. Carmeliet, M. Dewerchin

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

97 Citations (Scopus)

Abstract

OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.

Original language	English
Pages (from-to)	1695-1702
Journal	Artheriosclerosis, Thrombosis and Vascular Biology
Volume	30
Issue number	9
DOIs	https://doi.org/10.1161/ATVBAHA.110.203034
Publication status	Published - 2010

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Geudens, I., Herpers, R., Hermans, K., Segura, I., Ruiz de Almodovar, C., Bussmann, J., De Smet, F., Vandevelde, W., Hogan, B. M., Siekmann, A., Claes, F., Moore, J., Pistocchi, A. S., Loges, S., Mazzone, M., Mariggi, G., Bruyere, F., Cotelli, F., Kerjaschki, D., ... Dewerchin, M. (2010). Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish. Artheriosclerosis, Thrombosis and Vascular Biology, 30(9), 1695-1702. https://doi.org/10.1161/ATVBAHA.110.203034

@article{4b17c34d932e493c959b383d9b7df4d5,

title = "Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish",

abstract = "OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.",

author = "I. Geudens and R. Herpers and K. Hermans and I. Segura and {Ruiz de Almodovar}, C. and J. Bussmann and {De Smet}, F. and W. Vandevelde and B.M. Hogan and A. Siekmann and F. Claes and J. Moore and A.S. Pistocchi and S. Loges and M. Mazzone and G. Mariggi and F. Bruyere and F. Cotelli and D. Kerjaschki and A. Noel and J.M. Foidart and H. Gerhardt and A. Ny and T. Langenberg and N. Lawson and H.J. Duckers and S. Schulte-Merker and P. Carmeliet and M. Dewerchin",

note = "Reporting year: 2010",

year = "2010",

doi = "10.1161/ATVBAHA.110.203034",

language = "English",

volume = "30",

pages = "1695--1702",

journal = "Artheriosclerosis, Thrombosis and Vascular Biology",

issn = "1079-5642",

publisher = "Lippincott Williams & Wilkins",

number = "9",

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Geudens, I, Herpers, R, Hermans, K, Segura, I, Ruiz de Almodovar, C, Bussmann, J, De Smet, F, Vandevelde, W, Hogan, BM, Siekmann, A, Claes, F, Moore, J, Pistocchi, AS, Loges, S, Mazzone, M, Mariggi, G, Bruyere, F, Cotelli, F, Kerjaschki, D, Noel, A, Foidart, JM, Gerhardt, H, Ny, A, Langenberg, T, Lawson, N, Duckers, HJ, Schulte-Merker, S, Carmeliet, P & Dewerchin, M 2010, 'Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish', Artheriosclerosis, Thrombosis and Vascular Biology, vol. 30, no. 9, pp. 1695-1702. https://doi.org/10.1161/ATVBAHA.110.203034

TY - JOUR

T1 - Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

AU - Geudens, I.

AU - Herpers, R.

AU - Hermans, K.

AU - Segura, I.

AU - Ruiz de Almodovar, C.

AU - Bussmann, J.

AU - De Smet, F.

AU - Vandevelde, W.

AU - Hogan, B.M.

AU - Siekmann, A.

AU - Claes, F.

AU - Moore, J.

AU - Pistocchi, A.S.

AU - Loges, S.

AU - Mazzone, M.

AU - Mariggi, G.

AU - Bruyere, F.

AU - Cotelli, F.

AU - Kerjaschki, D.

AU - Noel, A.

AU - Foidart, J.M.

AU - Gerhardt, H.

AU - Ny, A.

AU - Langenberg, T.

AU - Lawson, N.

AU - Duckers, H.J.

AU - Schulte-Merker, S.

AU - Carmeliet, P.

AU - Dewerchin, M.

N1 - Reporting year: 2010

PY - 2010

Y1 - 2010

N2 - OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.

AB - OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.

U2 - 10.1161/ATVBAHA.110.203034

DO - 10.1161/ATVBAHA.110.203034

M3 - Article

SN - 1079-5642

VL - 30

SP - 1695

EP - 1702

JO - Artheriosclerosis, Thrombosis and Vascular Biology

JF - Artheriosclerosis, Thrombosis and Vascular Biology

IS - 9

ER -

Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

Abstract

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