Sample preparation for small RNA massive parallel sequencing

TY - JOUR

T1 - Sample preparation for small RNA massive parallel sequencing

AU - Gommans, W.M.

AU - Berezikov, E.

N1 - Reporting year: 2012

PY - 2012

Y1 - 2012

N2 - High-throughput sequencing has allowed for a comprehensive small RNA (sRNA) expression analysis of numerous tissues in a diverse set of organisms. The computational analysis of the millions of generated sequencing reads has led to the discovery of novel miRNAs and other sRNA species, and resulted in a better understanding of the roles these sRNAs play in development and disease. This chapter describes the generation of sRNA deep-sequencing libraries for the Illumina massively parallel sequencing platform by using a cloning method that anneals specific RNA sequences to the 5'- and 3'-ends of the sRNA molecules.

AB - High-throughput sequencing has allowed for a comprehensive small RNA (sRNA) expression analysis of numerous tissues in a diverse set of organisms. The computational analysis of the millions of generated sequencing reads has led to the discovery of novel miRNAs and other sRNA species, and resulted in a better understanding of the roles these sRNAs play in development and disease. This chapter describes the generation of sRNA deep-sequencing libraries for the Illumina massively parallel sequencing platform by using a cloning method that anneals specific RNA sequences to the 5'- and 3'-ends of the sRNA molecules.

U2 - 10.1007/978-1-61779-292-2_10

DO - 10.1007/978-1-61779-292-2_10

M3 - Article

SN - 1064-3745

VL - 786

SP - 167

EP - 178

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -