Single-cell genome sequencing of human neurons identifies somatic point mutation and indel enrichment in regulatory elements

Lovelace J Luquette, Michael B Miller, Zinan Zhou, Craig L Bohrson, Yifan Zhao, Hu Jin, Doga Gulhan, Javier Ganz, Sara Bizzotto, Samantha Kirkham, Tino Hochepied, Claude Libert, Alon Galor, Junho Kim, Michael A Lodato, Juan I Garaycoechea, Charles Gawad, Jay West, Christopher A Walsh, Peter J Park

Research output: Contribution to journal/periodicalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

Accurate somatic mutation detection from single-cell DNA sequencing is challenging due to amplification-related artifacts. To reduce this artifact burden, an improved amplification technique, primary template-directed amplification (PTA), was recently introduced. We analyzed whole-genome sequencing data from 52 PTA-amplified single neurons using SCAN2, a new genotyper we developed to leverage mutation signatures and allele balance in identifying somatic single-nucleotide variants (SNVs) and small insertions and deletions (indels) in PTA data. Our analysis confirms an increase in nonclonal somatic mutation in single neurons with age, but revises the estimated rate of this accumulation to 16 SNVs per year. We also identify artifacts in other amplification methods. Most importantly, we show that somatic indels increase by at least three per year per neuron and are enriched in functional regions of the genome such as enhancers and promoters. Our data suggest that indels in gene-regulatory elements have a considerable effect on genome integrity in human neurons.

Original languageEnglish
Pages (from-to)1564-1571
Number of pages8
JournalNature Genetics
Volume54
Issue number10
DOIs
Publication statusPublished - Oct 2022

Keywords

  • Genome, Human/genetics
  • High-Throughput Nucleotide Sequencing/methods
  • Humans
  • INDEL Mutation/genetics
  • Neurons
  • Nucleotides
  • Point Mutation
  • Polymorphism, Single Nucleotide/genetics
  • Single-Cell Analysis

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