Singulisphaera rosea sp. nov., a planctomycete from acidic Sphagnum peat, and emended description of the genus Singulisphaera

I.S. Kulichevskaya, E.N. Detkova, P.L.E. Bodelier, W.I.C. Rijpstra, J.S. Sinninghe Damsté, S.N. Dedysh

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A novel species, Singulisphaera rosea sp. nov., is proposed for aerobic, pink-pigmented, budding bacterium isolated from an acidic Sphagnum peat bog of northwestern Russia. This bacterium, designated strain S26T, has non-motile, spherical cells that occur singly, in pairs or in short chains and attach to surfaces by means of a holdfast material. Novel isolate is moderately acidophilic, mesophilic organism capable of growth at pH values between 3.2 and 7.1 (with an optimum at pH 4.8-5.0) and at temperatures between 4 and 33°C (with an optimum at 20-26°C). Most sugars, several organic acids and polyalcohols are the preferred growth substrates. The major fatty acids are C16:0, C18:1ω9c and C18:2ω6c,12c. The major neutral lipids are n-C31:9 hydrocarbon and squalene; the polar lipids are phosphatidylglycerol, phosphatidylcholine, and an unknown group of intact polar lipids. The G+C content of the DNA is 62.2 mol%. 16S rRNA gene sequence analysis showed that strain S26T is a member of the order Planctomycetales. Among taxonomically characterized representatives of this order, the highest sequence similarity (95.1-95.2%) was observed with non-filamentous, peat-inhabiting planctomycete Singulisphaera acidiphila. However, compared with S. acidiphila, the novel isolate is pigmented, displays significant differences in substrate utilization pattern, has greater tolerance of acidic conditions and contains the C16:1ω9c fatty acid. These differences indicate that strain S26T (=DSM 23044T = VKM B-2599T) represents a novel species of the genus Singulisphaera, Singulisphaera rosea sp. nov.
Original languageEnglish
Pages (from-to)118-123
JournalInternational Journal of Systematic and Evolutionary Microbiology
Issue number1
Publication statusPublished - 2012


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