• grasselli

    Final published version, 3 MB, PDF-document


  • Giorgio Grasselli
  • Henk-Jan Boele
  • Heather K Titley
  • Nora Bradford
  • Lisa van Beers
  • Lindsey Jay
  • Gerco C Beekhof
  • Silas E Busch
  • Chris I de Zeeuw
  • Martijn Schonewille
  • Christian Hansel

Neurons store information by changing synaptic input weights. In addition, they can adjust their membrane excitability to alter spike output. Here, we demonstrate a role of such "intrinsic plasticity" in behavioral learning in a mouse model that allows us to detect specific consequences of absent excitability modulation. Mice with a Purkinje-cell-specific knockout (KO) of the calcium-activated K+ channel SK2 (L7-SK2) show intact vestibulo-ocular reflex (VOR) gain adaptation but impaired eyeblink conditioning (EBC), which relies on the ability to establish associations between stimuli, with the eyelid closure itself depending on a transient suppression of spike firing. In these mice, the intrinsic plasticity of Purkinje cells is prevented without affecting long-term depression or potentiation at their parallel fiber (PF) input. In contrast to the typical spike pattern of EBC-supporting zebrin-negative Purkinje cells, L7-SK2 neurons show reduced background spiking but enhanced excitability. Thus, SK2 plasticity and excitability modulation are essential for specific forms of motor learning.

Original languageEnglish
Pages (from-to)e3000596
JournalPLoS Biology
Issue number1
Publication statusPublished - 2020

ID: 12860160