Soil inoculation alters the endosphere microbiome of chrysanthemum roots and leaves

N. Pangesti, A.M. Pineda, Emilia Hannula, T.M. Bezemer (Corresponding author)

Research output: Contribution to journal/periodicalArticleScientificpeer-review

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Abstract

Aims
This study examines how inoculation with live soil influences the assembly of the endosphere microbiome of leaves and roots of chrysanthemum.

Methods
Sterilized soil was inoculated with 10% soil in which grasses had grown. Chrysanthemum was planted in these soils and control plants were grown in 100% sterilized soil. All plants were exposed to thrips, and leaves and roots were collected from inoculated and control plants that experienced high and low thrips damage. DNA was extracted and the bacterial and fungal community inside roots and leaves was determined using Illumina sequencing.

Results
Inoculation increased bacterial diversity in root but not in leaf tissues. The endosphere of both roots and leaves was dominated by Pseudomonadaceae. In leaves, the relative abundance of Pseudomonadaceae was higher in inoculated than in control plants, whereas this was opposite in roots. Leaves contained more rare bacterial families than roots. The number of fungal reads was very low and the endopshere did not differ between plants with high or low thrips damage.

Conclusions
Bacterial communities inside chrysanthemum root and leaf tissues differ considerably. Soil inoculation with entire microbiomes can be used to change root and foliar bacterial microbiomes and this is particularly effective in root tissues.
Original languageEnglish
JournalPlant and Soil
Volumein press
DOIs
Publication statusE-pub ahead of print - 2020

Keywords

  • NIOO
  • Plan_S-Compliant_OA

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