Species diversity of Pseudocercospora from Far East Asia

TY - JOUR

T1 - Species diversity of Pseudocercospora from Far East Asia

AU - Nakashima, Chiharu

AU - Motohashi, Keiichi

AU - Chen, Chi-Yu

AU - Groenewald, Johannes Z.

AU - Crous, Pedro W.

PY - 2016/12

Y1 - 2016/12

N2 - This study reflects on the monophyly of, and species diversity within, the genus Pseudocercospora in Far East Asia. Morphological characteristics and phylogenetic analyses of Pseudocercospora species were based on type specimens and ex-type cultures, which were collected from Japan and Taiwan. A phylogenetic tree was generated from multi-locus DNA sequence data of the internal transcribed spacer regions of the nrDNA cistron (ITS), partial actin (actA), and partial translation elongation factor 1-alpha (tef1), as well as the partial DNA-directed RNA polymerase II second largest subunit (rpb2). Based on these results, Pseudocercospora amelanchieris on Amelanchier and Ps. iwakiensis on Ilex were newly described from Japan, and a further 22 types (incl. two neo-, five lecto-, and 15 epitypes), were designated. The genus Pseudocercospora as presently circumscribed was found to be monophyletic, while the secondary barcodes, actA, tef1, and rpb2 were shown to be well suited to delimitate species within the genus.

AB - This study reflects on the monophyly of, and species diversity within, the genus Pseudocercospora in Far East Asia. Morphological characteristics and phylogenetic analyses of Pseudocercospora species were based on type specimens and ex-type cultures, which were collected from Japan and Taiwan. A phylogenetic tree was generated from multi-locus DNA sequence data of the internal transcribed spacer regions of the nrDNA cistron (ITS), partial actin (actA), and partial translation elongation factor 1-alpha (tef1), as well as the partial DNA-directed RNA polymerase II second largest subunit (rpb2). Based on these results, Pseudocercospora amelanchieris on Amelanchier and Ps. iwakiensis on Ilex were newly described from Japan, and a further 22 types (incl. two neo-, five lecto-, and 15 epitypes), were designated. The genus Pseudocercospora as presently circumscribed was found to be monophyletic, while the secondary barcodes, actA, tef1, and rpb2 were shown to be well suited to delimitate species within the genus.

KW - Epitypification

KW - New species

KW - Phylogenetic relationship

KW - Species criteria

KW - Taxonomy

U2 - 10.1007/s11557-016-1231-7

DO - 10.1007/s11557-016-1231-7

M3 - Article

SN - 1861-8952

VL - 15

SP - 1093

EP - 1117

JO - Mycological Progress

JF - Mycological Progress

IS - 10

ER -