Species of Cercospora associated with grey leaf spot of maize

P.W. Crous; J.Z. Groenewald; M. Groenewald; P. Caldwell; U. Braun; T.C. Harrington

doi:10.3114/sim.55.1.189

Species of Cercospora associated with grey leaf spot of maize

P.W. Crous, J.Z. Groenewald, M. Groenewald, P. Caldwell, U. Braun, T.C. Harrington

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Abstract

Grey leaf spot is a serious yield-reducing disease of maize (Zea mays) in many parts of the world where this crop is cultivated. The causal organism associated with the disease is Cercospora zeae-maydis. Two potential sibling species have been recognized as Groups I and II. The DNA sequences for the internal transcribed spacers (ITS1 & ITS2), the 5.8S rRNA gene, elongation factor 1-{alpha}, histone H3, actin and calmodulin gene regions suggest that Groups I and II are two distinct species. Furthermore, Cercospora zeae-maydis (Group I) can be distinguished from C. zeina sp. nov. (Group II) by its faster growth rate on artificial media, the ability to produce cercosporin, longer conidiophores, and broadly fusiform conidia. A PCR-based test that distinguishes the two species was developed using species-specific primers designed from the histone H3 gene.

Original language	English
Pages (from-to)	189-197
Journal	Studies in Mycology
Volume	55
DOIs	https://doi.org/10.3114/sim.55.1.189
Publication status	Published - 2006

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title = "Species of Cercospora associated with grey leaf spot of maize",

abstract = "Grey leaf spot is a serious yield-reducing disease of maize (Zea mays) in many parts of the world where this crop is cultivated. The causal organism associated with the disease is Cercospora zeae-maydis. Two potential sibling species have been recognized as Groups I and II. The DNA sequences for the internal transcribed spacers (ITS1 & ITS2), the 5.8S rRNA gene, elongation factor 1-{alpha}, histone H3, actin and calmodulin gene regions suggest that Groups I and II are two distinct species. Furthermore, Cercospora zeae-maydis (Group I) can be distinguished from C. zeina sp. nov. (Group II) by its faster growth rate on artificial media, the ability to produce cercosporin, longer conidiophores, and broadly fusiform conidia. A PCR-based test that distinguishes the two species was developed using species-specific primers designed from the histone H3 gene.",

author = "P.W. Crous and J.Z. Groenewald and M. Groenewald and P. Caldwell and U. Braun and T.C. Harrington",

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T1 - Species of Cercospora associated with grey leaf spot of maize

AU - Crous, P.W.

AU - Groenewald, J.Z.

AU - Groenewald, M.

AU - Caldwell, P.

AU - Braun, U.

AU - Harrington, T.C.

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PY - 2006

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N2 - Grey leaf spot is a serious yield-reducing disease of maize (Zea mays) in many parts of the world where this crop is cultivated. The causal organism associated with the disease is Cercospora zeae-maydis. Two potential sibling species have been recognized as Groups I and II. The DNA sequences for the internal transcribed spacers (ITS1 & ITS2), the 5.8S rRNA gene, elongation factor 1-{alpha}, histone H3, actin and calmodulin gene regions suggest that Groups I and II are two distinct species. Furthermore, Cercospora zeae-maydis (Group I) can be distinguished from C. zeina sp. nov. (Group II) by its faster growth rate on artificial media, the ability to produce cercosporin, longer conidiophores, and broadly fusiform conidia. A PCR-based test that distinguishes the two species was developed using species-specific primers designed from the histone H3 gene.

AB - Grey leaf spot is a serious yield-reducing disease of maize (Zea mays) in many parts of the world where this crop is cultivated. The causal organism associated with the disease is Cercospora zeae-maydis. Two potential sibling species have been recognized as Groups I and II. The DNA sequences for the internal transcribed spacers (ITS1 & ITS2), the 5.8S rRNA gene, elongation factor 1-{alpha}, histone H3, actin and calmodulin gene regions suggest that Groups I and II are two distinct species. Furthermore, Cercospora zeae-maydis (Group I) can be distinguished from C. zeina sp. nov. (Group II) by its faster growth rate on artificial media, the ability to produce cercosporin, longer conidiophores, and broadly fusiform conidia. A PCR-based test that distinguishes the two species was developed using species-specific primers designed from the histone H3 gene.

U2 - 10.3114/sim.55.1.189

DO - 10.3114/sim.55.1.189

M3 - Article

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SP - 189

EP - 197

JO - Studies in Mycology

JF - Studies in Mycology

ER -

Species of Cercospora associated with grey leaf spot of maize

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