Suhomyces drosophilae sp. nov., isolated from Drosophila flies feeding on a stinkhorn mushroom

Reshma Jadhav, Snigdha Tiwari, Rameshwar Avchar, Marizeth Groenewald, Abhishek Baghela

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

The majority of Suhomyces species have been isolated from fungus-feeding insects and particularly from the gut of beetles. In the present study, seven yeast strains were isolated from the gut of Drosophila species feeding on gleba, the spore-bearing inner mass, of a stinkhorn mushroom belonging to the family Phallaceae. Based on phenotypic, biochemical characterization and sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer (ITS) region, two of these yeast strains, DGY3 and DGY4, represented a novel species of the genus Suhomyces. The novel species is closely related to an undescribed species of Candida ST-370 (DQ404513) and with Suhomyces canberraensis, wherein, the novel species differs from S. canberraensis by 40 nucleotide substitutions and three gaps (7.7 % sequence variation) in the D1/D2 region and 50 nucleotide substitutions and seven gaps (13.7 % sequence variation) in the ITS region. Several morphological and physiological differences were also observed between S. canberraensis and the strains obtained during this study. These data support the proposal of Suhomyces drosophilae as a novel species, with DGY3T as the holotype and CBS 16329T and MCC 1871T as ex-type strains.

Original languageEnglish
Pages (from-to)4908-4913
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number9
DOIs
Publication statusPublished - Sep 2020

Keywords

  • Agaricales
  • Animals
  • DNA, Fungal/genetics
  • DNA, Ribosomal Spacer/genetics
  • Drosophila/microbiology
  • India
  • Mycological Typing Techniques
  • Phylogeny
  • Saccharomycetales/classification

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