TY - JOUR
T1 - Symptomatic citrus trees reveal a new pathogenic lineage in fusarium and two new neocosmospora species
AU - Sandoval-Denis, M.
AU - Guarnaccia, V.
AU - Polizzi, G.
AU - Crous, P. W.
N1 - Funding Information:
2 T: Ex-type, ET: Ex-epitype, NT: Ex-neotype. ATCC: American Type Culture Collection, Manasas, VA, USA; BBA: Biologische Bundesanstalt für Land-und Forstwirtschaft, Berlin-Dahlem, Germany; CBS: Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands; CDC: Centers for Disease Control and Prevention, Atlanta, GA, USA; CML: Coleção Micológica de Lavras, MG, Brazil; F: Laboratory of Zhi-Min Cao, Northwest A&F University, Shaanxi, China; FMR: Facultat de Medicina i Ciències de la Salut, Reus, Spain; FRC: Fusarium Research Center, University Park, PA, USA; IMI: CABI Biosciences, Egham, Surrey, England; LEMM: Laboratorio Especializado de Micología Médica, Bogotá, Colombia; NRRL: Agricultural Research Service Culture Collection, NCAUR-ARS-USDA, Peoria, IL, USA; UTHSC: Fungus Testing Labora-tory, Department of Pathology, University of Texas Health Science Center, San Antonio, USA; RBG: Royal Botanic Gardens Trust, Sydney, New South Wales, Australia. 3 CAM: Calmodulin; EF-1α: Translation elongation factor 1-alpha; ITS: Internal transcribed spacer regions of the rDNA and 5.8S region; LSU: Partial large subunit of the rDNA; RPB1: RNA polymerase largest subunit; RPB2: RNA polymerase second largest subunit; TUB: Beta-tubulin. Sequences generated in this study appear in bold.
Publisher Copyright:
© 2017 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/6
Y1 - 2018/6
N2 - The diversity of fusaria in symptomatic Citrus trees in Greece, Italy and Spain was evaluated using morphological and molecular multi-locus analyses based on fragments of the calmodulin (CAM), intergenic spacer region of the rDNA (IGS), internal transcribed spacer region of the rDNA (ITS), large subunit of the rDNA (LSU), RNA polymerase largest subunit (RPB1), RNA polymerase second largest subunit (RPB2), translation elongation factor 1-alpha (EF-1α) and beta-tubulin (TUB) genes. A total of 11 species (six Fusarium spp., and five Neocosmospora spp.) were isolated from dry root rot, crown, trunk or twig canker or twig dieback of citrus trees. The most commonly isolated species were Fusarium sarcochroum, F. oxysporum and Neocosmospora solani. Three new Fusarium species are described, i.e., F. citricola and F. salinense belonging to the newly described F. citricola species complex; and F. siculi belonging to the F. fujikuroi species complex. Results of pathogenicity tests showed this new complex to include prominent canker causing agents affecting several Citrus spp. In addition, two new species are described in Neocosmospora, named N. croci and N. macrospora, the latter species being clearly differentiated from most members of this genus by producing large, up to nine-septate sporodochial conidia.
AB - The diversity of fusaria in symptomatic Citrus trees in Greece, Italy and Spain was evaluated using morphological and molecular multi-locus analyses based on fragments of the calmodulin (CAM), intergenic spacer region of the rDNA (IGS), internal transcribed spacer region of the rDNA (ITS), large subunit of the rDNA (LSU), RNA polymerase largest subunit (RPB1), RNA polymerase second largest subunit (RPB2), translation elongation factor 1-alpha (EF-1α) and beta-tubulin (TUB) genes. A total of 11 species (six Fusarium spp., and five Neocosmospora spp.) were isolated from dry root rot, crown, trunk or twig canker or twig dieback of citrus trees. The most commonly isolated species were Fusarium sarcochroum, F. oxysporum and Neocosmospora solani. Three new Fusarium species are described, i.e., F. citricola and F. salinense belonging to the newly described F. citricola species complex; and F. siculi belonging to the F. fujikuroi species complex. Results of pathogenicity tests showed this new complex to include prominent canker causing agents affecting several Citrus spp. In addition, two new species are described in Neocosmospora, named N. croci and N. macrospora, the latter species being clearly differentiated from most members of this genus by producing large, up to nine-septate sporodochial conidia.
KW - Citrus canker
KW - Citrus dieback
KW - Morphology
KW - Multigene phylogeny
KW - Systematics
UR - http://www.scopus.com/inward/record.url?scp=85021695567&partnerID=8YFLogxK
U2 - 10.3767/persoonia.2018.40.01
DO - 10.3767/persoonia.2018.40.01
M3 - Article
AN - SCOPUS:85021695567
SN - 0031-5850
VL - 40
SP - 1
EP - 25
JO - Persoonia: Molecular Phylogeny and Evolution of Fungi
JF - Persoonia: Molecular Phylogeny and Evolution of Fungi
ER -