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The analysis of pyrrolizidine alkaloids in Jacobaea vulgaris; a comparison of extraction and detection methods. / Joosten, L.; Mulder, P.P.J.; Vrieling, K.; Van Veen, J.A.; Klinkhamer, P.G.L.

In: Phytochemical Analysis, Vol. 21, No. 2, 2010, p. 197-204.

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Joosten, L. ; Mulder, P.P.J. ; Vrieling, K. ; Van Veen, J.A. ; Klinkhamer, P.G.L. / The analysis of pyrrolizidine alkaloids in Jacobaea vulgaris; a comparison of extraction and detection methods. In: Phytochemical Analysis. 2010 ; Vol. 21, No. 2. pp. 197-204.

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@article{08d8b801924340a6888f043e55b7935b,
title = "The analysis of pyrrolizidine alkaloids in Jacobaea vulgaris; a comparison of extraction and detection methods",
abstract = "Introduction - Pyrrolizidine alkaloids (PAs) serve an important function in plant defence. Objective - To compare different extraction methods and detection techniques, namely gas chromatography with nitrogen phosphorus detection (GC-NPD) and liquid chromatography tandem mass spectrometry (LC-MS/MS) with quadrupole analysers for analysing PAs in Jacobaea vulgaris. Methodology - Both formic acid and sulfuric acid were tested for PA extraction from dry plant material. For GC-NPD, reduction is required to transform PA N-oxides into tertiary amines. Zinc and sodium metabisulfite were compared as reducing agents. Results - The lowest PA concentration measured with GC-NPD was approximately 0.03 mg/g and with LC-MS/MS 0.002 mg/g. The detection of major PAs by both techniques was comparable but a number of minor PAs were not detected by GC-NPD. With the LC-MS/MS procedure higher concentrations were found in plant extracts, indicating that losses may have occurred during the sample preparation for the GC-NPD method. Zinc proved a more effective reducing agent than sodium metabisulfite. The sample preparation Conclusions - In terms of sensitivity and discrimination, formic acid extraction in combination with LC-MS/MS detection is the method of choice for analysing PAs (both free and N-oxides forms) in plant material.",
author = "L. Joosten and P.P.J. Mulder and K. Vrieling and {Van Veen}, J.A. and P.G.L. Klinkhamer",
note = "Reporting year: 2010 Metis note: 4760;CTE; ME; file:///L:\EndnoteDatabases\NIOOPUB\pdfs\PDFS2010\Joosten_ea_4760.pdf",
year = "2010",
doi = "10.1002/pca.1183",
language = "English",
volume = "21",
pages = "197--204",
journal = "Phytochemical Analysis",
issn = "0958-0344",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - The analysis of pyrrolizidine alkaloids in Jacobaea vulgaris; a comparison of extraction and detection methods

AU - Joosten, L.

AU - Mulder, P.P.J.

AU - Vrieling, K.

AU - Van Veen, J.A.

AU - Klinkhamer, P.G.L.

N1 - Reporting year: 2010 Metis note: 4760;CTE; ME; file:///L:\EndnoteDatabases\NIOOPUB\pdfs\PDFS2010\Joosten_ea_4760.pdf

PY - 2010

Y1 - 2010

N2 - Introduction - Pyrrolizidine alkaloids (PAs) serve an important function in plant defence. Objective - To compare different extraction methods and detection techniques, namely gas chromatography with nitrogen phosphorus detection (GC-NPD) and liquid chromatography tandem mass spectrometry (LC-MS/MS) with quadrupole analysers for analysing PAs in Jacobaea vulgaris. Methodology - Both formic acid and sulfuric acid were tested for PA extraction from dry plant material. For GC-NPD, reduction is required to transform PA N-oxides into tertiary amines. Zinc and sodium metabisulfite were compared as reducing agents. Results - The lowest PA concentration measured with GC-NPD was approximately 0.03 mg/g and with LC-MS/MS 0.002 mg/g. The detection of major PAs by both techniques was comparable but a number of minor PAs were not detected by GC-NPD. With the LC-MS/MS procedure higher concentrations were found in plant extracts, indicating that losses may have occurred during the sample preparation for the GC-NPD method. Zinc proved a more effective reducing agent than sodium metabisulfite. The sample preparation Conclusions - In terms of sensitivity and discrimination, formic acid extraction in combination with LC-MS/MS detection is the method of choice for analysing PAs (both free and N-oxides forms) in plant material.

AB - Introduction - Pyrrolizidine alkaloids (PAs) serve an important function in plant defence. Objective - To compare different extraction methods and detection techniques, namely gas chromatography with nitrogen phosphorus detection (GC-NPD) and liquid chromatography tandem mass spectrometry (LC-MS/MS) with quadrupole analysers for analysing PAs in Jacobaea vulgaris. Methodology - Both formic acid and sulfuric acid were tested for PA extraction from dry plant material. For GC-NPD, reduction is required to transform PA N-oxides into tertiary amines. Zinc and sodium metabisulfite were compared as reducing agents. Results - The lowest PA concentration measured with GC-NPD was approximately 0.03 mg/g and with LC-MS/MS 0.002 mg/g. The detection of major PAs by both techniques was comparable but a number of minor PAs were not detected by GC-NPD. With the LC-MS/MS procedure higher concentrations were found in plant extracts, indicating that losses may have occurred during the sample preparation for the GC-NPD method. Zinc proved a more effective reducing agent than sodium metabisulfite. The sample preparation Conclusions - In terms of sensitivity and discrimination, formic acid extraction in combination with LC-MS/MS detection is the method of choice for analysing PAs (both free and N-oxides forms) in plant material.

U2 - 10.1002/pca.1183

DO - 10.1002/pca.1183

M3 - Article

VL - 21

SP - 197

EP - 204

JO - Phytochemical Analysis

JF - Phytochemical Analysis

SN - 0958-0344

IS - 2

ER -

ID: 351999