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The Development of a Genome Wide SNP Set for the Barnacle Goose Branta leucopsis. / Jonker, R.M.; Zhang, Q.; Van Hooft, P.; Loonen, M.J.J.E.; Van der Jeugd, H.P.; Crooijmans, R.P.M.A.; Groenen, M.A.M.; Prins, H.H.T.; Kraus, R.H.S.

In: PLoS One, Vol. 7, No. 7, e38412, 2012.

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Harvard

Jonker, RM, Zhang, Q, Van Hooft, P, Loonen, MJJE, Van der Jeugd, HP, Crooijmans, RPMA, Groenen, MAM, Prins, HHT & Kraus, RHS 2012, 'The Development of a Genome Wide SNP Set for the Barnacle Goose Branta leucopsis' PLoS One, vol. 7, no. 7, e38412. https://doi.org/10.1371/journal.pone.0038412

APA

Jonker, R. M., Zhang, Q., Van Hooft, P., Loonen, M. J. J. E., Van der Jeugd, H. P., Crooijmans, R. P. M. A., ... Kraus, R. H. S. (2012). The Development of a Genome Wide SNP Set for the Barnacle Goose Branta leucopsis. PLoS One, 7(7), [e38412]. https://doi.org/10.1371/journal.pone.0038412

Vancouver

Jonker RM, Zhang Q, Van Hooft P, Loonen MJJE, Van der Jeugd HP, Crooijmans RPMA et al. The Development of a Genome Wide SNP Set for the Barnacle Goose Branta leucopsis. PLoS One. 2012;7(7). e38412. https://doi.org/10.1371/journal.pone.0038412

Author

Jonker, R.M. ; Zhang, Q. ; Van Hooft, P. ; Loonen, M.J.J.E. ; Van der Jeugd, H.P. ; Crooijmans, R.P.M.A. ; Groenen, M.A.M. ; Prins, H.H.T. ; Kraus, R.H.S. / The Development of a Genome Wide SNP Set for the Barnacle Goose Branta leucopsis. In: PLoS One. 2012 ; Vol. 7, No. 7.

BibTeX

@article{c9e815cc50ef40239dd867b0c16e8f6f,
title = "The Development of a Genome Wide SNP Set for the Barnacle Goose Branta leucopsis",
abstract = "Migratory birds are of particular interest for population genetics because of the high connectivity between habitats and populations. A high degree of connectivity requires using many genetic markers to achieve the required statistical power, and a genome wide SNP set can fit this purpose. Here we present the development of a genome wide SNP set for the Barnacle Goose Branta leucopsis, a model species for the study of bird migration. We used the genome of a different waterfowl species, Mallard Anas platyrhynchos, as a reference to align Barnacle Goose second generation sequence reads from an RRL library and detected 2188 SNPs genome wide. Furthermore, we used chimeric flanking sequences, merged from both Mallard and Barnacle Goose DNA sequence information, to create primers for validation by genotyping. Validation with a 384 SNP genotyping set resulted in 374 (97{\%}) successfully typed SNPs in the assay, of which 358 (96{\%}) were polymorphic. Additionally, we validated our SNPs on relatively old (30 years) museum samples, which resulted in a success rate of at least 80{\%}. This shows that museum samples could be used in standard SNP genotyping assays. Our study also shows that the genome of a related species can be used as reference to detect genome wide SNPs in birds, because genomes of birds are highly conserved. This is illustrated by the use of chimeric flanking sequences, which showed that the incorporation of flanking nucleotides from Mallard into Barnacle Goose sequences lead to equal genotyping performance when compared to flanking sequences solely composed of Barnacle Goose sequence.",
keywords = "international",
author = "R.M. Jonker and Q. Zhang and {Van Hooft}, P. and M.J.J.E. Loonen and {Van der Jeugd}, H.P. and R.P.M.A. Crooijmans and M.A.M. Groenen and H.H.T. Prins and R.H.S. Kraus",
note = "Reporting year: 2012 Metis note: 5331; WAG; VT",
year = "2012",
doi = "10.1371/journal.pone.0038412",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

RIS

TY - JOUR

T1 - The Development of a Genome Wide SNP Set for the Barnacle Goose Branta leucopsis

AU - Jonker, R.M.

AU - Zhang, Q.

AU - Van Hooft, P.

AU - Loonen, M.J.J.E.

AU - Van der Jeugd, H.P.

AU - Crooijmans, R.P.M.A.

AU - Groenen, M.A.M.

AU - Prins, H.H.T.

AU - Kraus, R.H.S.

N1 - Reporting year: 2012 Metis note: 5331; WAG; VT

PY - 2012

Y1 - 2012

N2 - Migratory birds are of particular interest for population genetics because of the high connectivity between habitats and populations. A high degree of connectivity requires using many genetic markers to achieve the required statistical power, and a genome wide SNP set can fit this purpose. Here we present the development of a genome wide SNP set for the Barnacle Goose Branta leucopsis, a model species for the study of bird migration. We used the genome of a different waterfowl species, Mallard Anas platyrhynchos, as a reference to align Barnacle Goose second generation sequence reads from an RRL library and detected 2188 SNPs genome wide. Furthermore, we used chimeric flanking sequences, merged from both Mallard and Barnacle Goose DNA sequence information, to create primers for validation by genotyping. Validation with a 384 SNP genotyping set resulted in 374 (97%) successfully typed SNPs in the assay, of which 358 (96%) were polymorphic. Additionally, we validated our SNPs on relatively old (30 years) museum samples, which resulted in a success rate of at least 80%. This shows that museum samples could be used in standard SNP genotyping assays. Our study also shows that the genome of a related species can be used as reference to detect genome wide SNPs in birds, because genomes of birds are highly conserved. This is illustrated by the use of chimeric flanking sequences, which showed that the incorporation of flanking nucleotides from Mallard into Barnacle Goose sequences lead to equal genotyping performance when compared to flanking sequences solely composed of Barnacle Goose sequence.

AB - Migratory birds are of particular interest for population genetics because of the high connectivity between habitats and populations. A high degree of connectivity requires using many genetic markers to achieve the required statistical power, and a genome wide SNP set can fit this purpose. Here we present the development of a genome wide SNP set for the Barnacle Goose Branta leucopsis, a model species for the study of bird migration. We used the genome of a different waterfowl species, Mallard Anas platyrhynchos, as a reference to align Barnacle Goose second generation sequence reads from an RRL library and detected 2188 SNPs genome wide. Furthermore, we used chimeric flanking sequences, merged from both Mallard and Barnacle Goose DNA sequence information, to create primers for validation by genotyping. Validation with a 384 SNP genotyping set resulted in 374 (97%) successfully typed SNPs in the assay, of which 358 (96%) were polymorphic. Additionally, we validated our SNPs on relatively old (30 years) museum samples, which resulted in a success rate of at least 80%. This shows that museum samples could be used in standard SNP genotyping assays. Our study also shows that the genome of a related species can be used as reference to detect genome wide SNPs in birds, because genomes of birds are highly conserved. This is illustrated by the use of chimeric flanking sequences, which showed that the incorporation of flanking nucleotides from Mallard into Barnacle Goose sequences lead to equal genotyping performance when compared to flanking sequences solely composed of Barnacle Goose sequence.

KW - international

U2 - 10.1371/journal.pone.0038412

DO - 10.1371/journal.pone.0038412

M3 - Article

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 7

M1 - e38412

ER -

ID: 225012