TY - JOUR
T1 - The function of GORASPs in Golgi apparatus organization in vivo
AU - Grond, Rianne
AU - Veenendaal, Tineke
AU - Duran, Juan M
AU - Raote, Ishier
AU - van Es, Johan H
AU - Corstjens, Sebastiaan
AU - Delfgou, Laura
AU - El Haddouti, Benaissa
AU - Malhotra, Vivek
AU - Rabouille, Catherine
N1 - © 2020 Grond et al.
PY - 2020/9/7
Y1 - 2020/9/7
N2 - In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.
AB - In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.
U2 - 10.1083/jcb.202004191
DO - 10.1083/jcb.202004191
M3 - Article
C2 - 32573693
SN - 0021-9525
VL - 219
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 9
ER -