The function of GORASPs in Golgi apparatus organization in vivo

Rianne Grond, Tineke Veenendaal, Juan M Duran, Ishier Raote, Johan H van Es, Sebastiaan Corstjens, Laura Delfgou, Benaissa El Haddouti, Vivek Malhotra, Catherine Rabouille

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.

Original languageEnglish
JournalJournal of Cell Biology
Volume219
Issue number9
DOIs
Publication statusPublished - 07 Sep 2020

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