TY - JOUR
T1 - The genome sequence of the model ascomycete fungus Podospora anserina
AU - Espagne, Eric
AU - Lespinet, Olivier
AU - Malagnac, Fabienne
AU - Da Silva, Corinne
AU - Jaillon, Olivier
AU - Porcel, Betina M
AU - Couloux, Arnaud
AU - Aury, Jean-Marc
AU - Ségurens, Béatrice
AU - Poulain, Julie
AU - Anthouard, Véronique
AU - Grossetete, Sandrine
AU - Khalili, Hamid
AU - Coppin, Evelyne
AU - Déquard-Chablat, Michelle
AU - Picard, Marguerite
AU - Contamine, Véronique
AU - Arnaise, Sylvie
AU - Bourdais, Anne
AU - Berteaux-Lecellier, Véronique
AU - Gautheret, Daniel
AU - de Vries, Ronald P
AU - Battaglia, Evy
AU - Coutinho, Pedro M
AU - Danchin, Etienne Gj
AU - Henrissat, Bernard
AU - Khoury, Riyad El
AU - Sainsard-Chanet, Annie
AU - Boivin, Antoine
AU - Pinan-Lucarré, Bérangère
AU - Sellem, Carole H
AU - Debuchy, Robert
AU - Wincker, Patrick
AU - Weissenbach, Jean
AU - Silar, Philippe
PY - 2008
Y1 - 2008
N2 - BACKGROUND: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.RESULTS: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown.CONCLUSION: The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.
AB - BACKGROUND: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.RESULTS: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown.CONCLUSION: The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.
KW - Base Sequence
KW - Carbon
KW - Evolution, Molecular
KW - Expressed Sequence Tags
KW - Gene Duplication
KW - Genome, Fungal
KW - Molecular Sequence Data
KW - Neurospora crassa
KW - Podospora
U2 - 10.1186/gb-2008-9-5-r77
DO - 10.1186/gb-2008-9-5-r77
M3 - Article
C2 - 18460219
SN - 1474-760X
VL - 9
SP - R77
JO - Genome Biology
JF - Genome Biology
IS - 5
ER -