The genome sequence of the model ascomycete fungus Podospora anserina

Eric Espagne; Olivier Lespinet; Fabienne Malagnac; Corinne Da Silva; Olivier Jaillon; Betina M Porcel; Arnaud Couloux; Jean-Marc Aury; Béatrice Ségurens; Julie Poulain; Véronique Anthouard; Sandrine Grossetete; Hamid Khalili; Evelyne Coppin; Michelle Déquard-Chablat; Marguerite Picard; Véronique Contamine; Sylvie Arnaise; Anne Bourdais; Véronique Berteaux-Lecellier; Daniel Gautheret; Ronald P de Vries; Evy Battaglia; Pedro M Coutinho; Etienne Gj Danchin; Bernard Henrissat; Riyad El Khoury; Annie Sainsard-Chanet; Antoine Boivin; Bérangère Pinan-Lucarré; Carole H Sellem; Robert Debuchy; Patrick Wincker; Jean Weissenbach; Philippe Silar

doi:10.1186/gb-2008-9-5-r77

The genome sequence of the model ascomycete fungus Podospora anserina

Eric Espagne, Olivier Lespinet, Fabienne Malagnac, Corinne Da Silva, Olivier Jaillon, Betina M Porcel, Arnaud Couloux, Jean-Marc Aury, Béatrice Ségurens, Julie Poulain, Véronique Anthouard, Sandrine Grossetete, Hamid Khalili, Evelyne Coppin, Michelle Déquard-Chablat, Marguerite Picard, Véronique Contamine, Sylvie Arnaise, Anne Bourdais, Véronique Berteaux-LecellierDaniel Gautheret, Ronald P de Vries, Evy Battaglia, Pedro M Coutinho, Etienne Gj Danchin, Bernard Henrissat, Riyad El Khoury, Annie Sainsard-Chanet, Antoine Boivin, Bérangère Pinan-Lucarré, Carole H Sellem, Robert Debuchy, Patrick Wincker, Jean Weissenbach, Philippe Silar

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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Abstract

BACKGROUND: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.

RESULTS: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown.

CONCLUSION: The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.

Original language	English
Pages (from-to)	R77
Journal	Genome Biology
Volume	9
Issue number	5
DOIs	https://doi.org/10.1186/gb-2008-9-5-r77
Publication status	Published - 2008

Keywords

Base Sequence
Carbon
Evolution, Molecular
Expressed Sequence Tags
Gene Duplication
Genome, Fungal
Molecular Sequence Data
Neurospora crassa
Podospora

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Espagne, E., Lespinet, O., Malagnac, F., Da Silva, C., Jaillon, O., Porcel, B. M., Couloux, A., Aury, J.-M., Ségurens, B., Poulain, J., Anthouard, V., Grossetete, S., Khalili, H., Coppin, E., Déquard-Chablat, M., Picard, M., Contamine, V., Arnaise, S., Bourdais, A., ... Silar, P. (2008). The genome sequence of the model ascomycete fungus Podospora anserina. Genome Biology, 9(5), R77. https://doi.org/10.1186/gb-2008-9-5-r77

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title = "The genome sequence of the model ascomycete fungus Podospora anserina",

abstract = "BACKGROUND: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.RESULTS: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown.CONCLUSION: The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.",

keywords = "Base Sequence, Carbon, Evolution, Molecular, Expressed Sequence Tags, Gene Duplication, Genome, Fungal, Molecular Sequence Data, Neurospora crassa, Podospora",

author = "Eric Espagne and Olivier Lespinet and Fabienne Malagnac and {Da Silva}, Corinne and Olivier Jaillon and Porcel, {Betina M} and Arnaud Couloux and Jean-Marc Aury and B{\'e}atrice S{\'e}gurens and Julie Poulain and V{\'e}ronique Anthouard and Sandrine Grossetete and Hamid Khalili and Evelyne Coppin and Michelle D{\'e}quard-Chablat and Marguerite Picard and V{\'e}ronique Contamine and Sylvie Arnaise and Anne Bourdais and V{\'e}ronique Berteaux-Lecellier and Daniel Gautheret and {de Vries}, {Ronald P} and Evy Battaglia and Coutinho, {Pedro M} and Danchin, {Etienne Gj} and Bernard Henrissat and Khoury, {Riyad El} and Annie Sainsard-Chanet and Antoine Boivin and B{\'e}rang{\`e}re Pinan-Lucarr{\'e} and Sellem, {Carole H} and Robert Debuchy and Patrick Wincker and Jean Weissenbach and Philippe Silar",

year = "2008",

doi = "10.1186/gb-2008-9-5-r77",

language = "English",

volume = "9",

pages = "R77",

journal = "Genome Biology",

issn = "1474-760X",

publisher = "BioMed Central",

number = "5",

}

Espagne, E, Lespinet, O, Malagnac, F, Da Silva, C, Jaillon, O, Porcel, BM, Couloux, A, Aury, J-M, Ségurens, B, Poulain, J, Anthouard, V, Grossetete, S, Khalili, H, Coppin, E, Déquard-Chablat, M, Picard, M, Contamine, V, Arnaise, S, Bourdais, A, Berteaux-Lecellier, V, Gautheret, D, de Vries, RP, Battaglia, E, Coutinho, PM, Danchin, EG, Henrissat, B, Khoury, RE, Sainsard-Chanet, A, Boivin, A, Pinan-Lucarré, B, Sellem, CH, Debuchy, R, Wincker, P, Weissenbach, J & Silar, P 2008, 'The genome sequence of the model ascomycete fungus Podospora anserina', Genome Biology, vol. 9, no. 5, pp. R77. https://doi.org/10.1186/gb-2008-9-5-r77

TY - JOUR

T1 - The genome sequence of the model ascomycete fungus Podospora anserina

AU - Espagne, Eric

AU - Lespinet, Olivier

AU - Malagnac, Fabienne

AU - Da Silva, Corinne

AU - Jaillon, Olivier

AU - Porcel, Betina M

AU - Couloux, Arnaud

AU - Aury, Jean-Marc

AU - Ségurens, Béatrice

AU - Poulain, Julie

AU - Anthouard, Véronique

AU - Grossetete, Sandrine

AU - Khalili, Hamid

AU - Coppin, Evelyne

AU - Déquard-Chablat, Michelle

AU - Picard, Marguerite

AU - Contamine, Véronique

AU - Arnaise, Sylvie

AU - Bourdais, Anne

AU - Berteaux-Lecellier, Véronique

AU - Gautheret, Daniel

AU - de Vries, Ronald P

AU - Battaglia, Evy

AU - Coutinho, Pedro M

AU - Danchin, Etienne Gj

AU - Henrissat, Bernard

AU - Khoury, Riyad El

AU - Sainsard-Chanet, Annie

AU - Boivin, Antoine

AU - Pinan-Lucarré, Bérangère

AU - Sellem, Carole H

AU - Debuchy, Robert

AU - Wincker, Patrick

AU - Weissenbach, Jean

AU - Silar, Philippe

PY - 2008

Y1 - 2008

N2 - BACKGROUND: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.RESULTS: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown.CONCLUSION: The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.

AB - BACKGROUND: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.RESULTS: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown.CONCLUSION: The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.

KW - Base Sequence

KW - Carbon

KW - Evolution, Molecular

KW - Expressed Sequence Tags

KW - Gene Duplication

KW - Genome, Fungal

KW - Molecular Sequence Data

KW - Neurospora crassa

KW - Podospora

U2 - 10.1186/gb-2008-9-5-r77

DO - 10.1186/gb-2008-9-5-r77

M3 - Article

C2 - 18460219

SN - 1474-760X

VL - 9

SP - R77

JO - Genome Biology

JF - Genome Biology

IS - 5

ER -

The genome sequence of the model ascomycete fungus Podospora anserina

Abstract

Keywords

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