The microRNA-producing enzyme Dicer1 is essential for zebrafish development

E. Wienholds; M.J. Koudijs; F. van Eeden; E. Cuppen; R. Plasterk

doi:10.1038/ng1251

The microRNA-producing enzyme Dicer1 is essential for zebrafish development

E. Wienholds, M.J. Koudijs, F. van Eeden, E. Cuppen, R. Plasterk

Hubrecht Institute

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388 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

Original language	English
Pages (from-to)	217-218
Journal	Nature Genetics
Volume	35
Issue number	3
DOIs	https://doi.org/10.1038/ng1251
Publication status	Published - 2003

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10.1038/ng1251

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title = "The microRNA-producing enzyme Dicer1 is essential for zebrafish development",

abstract = "MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.",

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AU - Cuppen, E.

AU - Plasterk, R.

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AB - MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

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JO - Nature Genetics

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The microRNA-producing enzyme Dicer1 is essential for zebrafish development

Abstract

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