TY - JOUR
T1 - The secretome of
Agaricus bisporus
T2 - Temporal dynamics of plant polysaccharides and lignin degradation.
AU - Duran, Katharina
AU - Magnin, Joris
AU - America, Antoine H P
AU - Peng, Mao
AU - Hilgers, Roelant
AU - de Vries, Ronald P
AU - Baars, Johan J P
AU - van Berkel, Willem J H
AU - Kuyper, Thomas W
AU - Kabel, Mirjam A
N1 - © 2023 The Author(s).
PY - 2023/7/21
Y1 - 2023/7/21
N2 - Despite substantial lignocellulose conversion during mycelial growth, previous transcriptome and proteome studies have not yet revealed how secretomes from the edible mushroom
Agaricus bisporus develop and whether they modify lignin models
in vitro. To clarify these aspects,
A. bisporus secretomes collected throughout a 15-day industrial substrate production and from axenic lab-cultures were subjected to proteomics, and tested on polysaccharides and lignin models. Secretomes (day 6-15) comprised
A. bisporus endo-acting and substituent-removing glycoside hydrolases, whereas β-xylosidase and glucosidase activities gradually decreased. Laccases appeared from day 6 onwards. From day 10 onwards, many oxidoreductases were found, with numerous multicopper oxidases (MCO), aryl alcohol oxidases (AAO), glyoxal oxidases (GLOX), a manganese peroxidase (MnP), and unspecific peroxygenases (UPO). Secretomes modified dimeric lignin models, thereby catalyzing syringylglycerol-β-guaiacyl ether (SBG) cleavage, guaiacylglycerol-β-guaiacyl ether (GBG) polymerization, and non-phenolic veratrylglycerol-β-guaiacyl ether (VBG) oxidation. We explored
A. bisporus secretomes and insights obtained can help to better understand biomass valorization.
AB - Despite substantial lignocellulose conversion during mycelial growth, previous transcriptome and proteome studies have not yet revealed how secretomes from the edible mushroom
Agaricus bisporus develop and whether they modify lignin models
in vitro. To clarify these aspects,
A. bisporus secretomes collected throughout a 15-day industrial substrate production and from axenic lab-cultures were subjected to proteomics, and tested on polysaccharides and lignin models. Secretomes (day 6-15) comprised
A. bisporus endo-acting and substituent-removing glycoside hydrolases, whereas β-xylosidase and glucosidase activities gradually decreased. Laccases appeared from day 6 onwards. From day 10 onwards, many oxidoreductases were found, with numerous multicopper oxidases (MCO), aryl alcohol oxidases (AAO), glyoxal oxidases (GLOX), a manganese peroxidase (MnP), and unspecific peroxygenases (UPO). Secretomes modified dimeric lignin models, thereby catalyzing syringylglycerol-β-guaiacyl ether (SBG) cleavage, guaiacylglycerol-β-guaiacyl ether (GBG) polymerization, and non-phenolic veratrylglycerol-β-guaiacyl ether (VBG) oxidation. We explored
A. bisporus secretomes and insights obtained can help to better understand biomass valorization.
U2 - 10.1016/j.isci.2023.107087
DO - 10.1016/j.isci.2023.107087
M3 - Article
C2 - 37426348
SN - 2589-0042
VL - 26
SP - 107087
JO - iScience
JF - iScience
IS - 7
ER -