TY - JOUR
T1 - The Sugar Metabolic Model of
Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum.
AU - Li, Jiajia
AU - Chroumpi, Tania
AU - Garrigues, Sandra
AU - Kun, Roland S
AU - Meng, Jiali
AU - Salazar-Cerezo, Sonia
AU - Aguilar-Pontes, Maria Victoria
AU - Zhang, Yu
AU - Tejomurthula, Sravanthi
AU - Lipzen, Anna
AU - Ng, Vivian
AU - Clendinen, Chaevien S
AU - Tolić, Nikola
AU - Grigoriev, Igor V
AU - Tsang, Adrian
AU - Mäkelä, Miia R
AU - Snel, Berend
AU - Peng, Mao
AU - de Vries, Ronald P
PY - 2022/12/17
Y1 - 2022/12/17
N2 - Fungi play a critical role in the global carbon cycle by degrading plant polysaccharides to small sugars and metabolizing them as carbon and energy sources. We mapped the well-established sugar metabolic network of
Aspergillus niger to five taxonomically distant species (
Aspergillus nidulans,
Penicillium subrubescens,
Trichoderma reesei,
Phanerochaete chrysosporium and
Dichomitus squalens) using an orthology-based approach. The diversity of sugar metabolism correlates well with the taxonomic distance of the fungi. The pathways are highly conserved between the three studied Eurotiomycetes (
A. niger,
A. nidulans,
P. subrubescens). A higher level of diversity was observed between the
T. reesei and
A. niger, and even more so for the two Basidiomycetes. These results were confirmed by integrative analysis of transcriptome, proteome and metabolome, as well as growth profiles of the fungi growing on the corresponding sugars. In conclusion, the establishment of sugar pathway models in different fungi revealed the diversity of fungal sugar conversion and provided a valuable resource for the community, which would facilitate rational metabolic engineering of these fungi as microbial cell factories.
AB - Fungi play a critical role in the global carbon cycle by degrading plant polysaccharides to small sugars and metabolizing them as carbon and energy sources. We mapped the well-established sugar metabolic network of
Aspergillus niger to five taxonomically distant species (
Aspergillus nidulans,
Penicillium subrubescens,
Trichoderma reesei,
Phanerochaete chrysosporium and
Dichomitus squalens) using an orthology-based approach. The diversity of sugar metabolism correlates well with the taxonomic distance of the fungi. The pathways are highly conserved between the three studied Eurotiomycetes (
A. niger,
A. nidulans,
P. subrubescens). A higher level of diversity was observed between the
T. reesei and
A. niger, and even more so for the two Basidiomycetes. These results were confirmed by integrative analysis of transcriptome, proteome and metabolome, as well as growth profiles of the fungi growing on the corresponding sugars. In conclusion, the establishment of sugar pathway models in different fungi revealed the diversity of fungal sugar conversion and provided a valuable resource for the community, which would facilitate rational metabolic engineering of these fungi as microbial cell factories.
U2 - 10.3390/jof8121315
DO - 10.3390/jof8121315
M3 - Article
C2 - 36547648
SN - 2309-608X
VL - 8
JO - Journal of Fungi
JF - Journal of Fungi
IS - 12
ER -