The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of D-galacturonic acid from pectin

Ebru Alazi, Jing Niu, Joanna E Kowalczyk, Mao Peng, Maria Victoria Aguilar Pontes, Jan A L van Kan, Jaap Visser, Ronald P de Vries, Arthur F J Ram

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    56 Citations (Scopus)

    Abstract

    We identified the D-galacturonic acid (GA) responsive transcriptional activator GaaR of the saprotrophic fungus Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell and to induce the GA-catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA. This article is protected by copyright. All rights reserved.

    Original languageEnglish
    JournalFEBS Letters
    DOIs
    Publication statusPublished - 13 May 2016

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