The transcriptional activators AraR and XlnR from Aspergillus niger regulate expression of pentose catabolic and pentose phosphate pathway genes

Evy Battaglia, M. Zhou, Ronald P de Vries, J. van den Brink

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    29 Citations (Scopus)

    Abstract

    The pentose catabolic pathway (PCP) and the pentose phosphate pathway (PPP) are required for the conversion of pentose sugars in fungi and are linked via d-xylulose-5-phosphate. Previously, it was shown that the PCP is regulated by the transcriptional activators XlnR and AraR in Aspergillus niger. Here we assessed whether XlnR and AraR also regulate the PPP. Expression of two genes, rpiA and talB, was reduced in the ΔaraR/ΔxlnR strain and increased in the xylulokinase negative strain (xkiA1) on d-xylose and/or l-arabinose. Bioinformatic analysis of the 1 kb promoter regions of rpiA and talB showed the presence of putative XlnR binding sites. Combining all results in this study, it strongly suggests that these two PPP genes are under regulation of XlnR in A. niger.

    Original languageEnglish
    Pages (from-to)531-40
    Number of pages10
    JournalResearch in Microbiology
    Volume165
    Issue number7
    DOIs
    Publication statusPublished - Sept 2014

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