Three-dimensional organization of the glomeruli in the antennal lobe of the parasitoid wasps Cotesia glomerata and C-rubecula

H.M. Smid, M.A.K. Bleeker, J.J.A. van Loon, L.E.M. Vet

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    Two closely related parasitoid wasp species, Cotesia glomerata and C. rubecula, differ in their use of associative learning. To investigate the neural basis underlying these differences, it is necessary to describe the olfactory pathway of both wasp species. This paper focuses on the organization of the glomeruli in the antennal lobe. Glomeruli were stained by retrograde axon tracing of all axons in the antennal nerve and observed by confocal laser scanning microscopy. Stacks of optical sections were processed with AMIRA software, and 3D digital models of the glomeruli were produced. The combined use of 2D images and 3D surface models of the antennal lobes enabled the identification of a set of corresponding glomeruli in both wasp species. This offers unique opportunities for the study of subtle differences involved in synaptic plasticity that may occur at the glomerular level and factors regulating this plasticity [KEYWORDS: antennal lobe, glomerulus, confocal laser scanning microscopy, digital atlas, axonal tract tracing, Cotesia glomerata, C. rubecula (insecta)]
    Original languageEnglish
    Pages (from-to)237-248
    JournalCell and Tissue Research
    Volume312
    Issue number2
    DOIs
    Publication statusPublished - 2003

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