Three isothermal amplification techniques for rapid identification of Cladophialophora carrionii, an agent of human chromoblastomycosis

S. Deng, G Sybren de Hoog, Weihua Pan, Min Chen, Bert Gerrits van den Ende, Liyue Yang, Jiufeng Sun, Mohammad Javad Najafzadeh, Wanqing Liao, Ruoyu Li

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    In this study, we developed rapid and sensitive assays for the detection of Cladophialophora carrionii, a common agent of human chromoblastomycosis. The isothermal techniques evaluated were rolling-circle amplification (RCA), multiplex ligation-dependent probe amplification (MLPA), and loop-mediated isothermal amplification (LAMP). The probes for RCA and MLPA were designed with target sequences in the rDNA internal transcribed spacer gene (ITS) region, and LAMP primers were designed using the elongation factor 1α gene (EF1); these probes and primers specifically amplified DNA of isolates of the species. The three techniques were sufficiently specific and sensitive for discriminating target DNA of C. carrionii from that of related Cladophialophora species and other agents of chromoblastomycosis. RCA, MLPA, and LAMP are advantageous in their reliability and ease of operation compared to standard PCR and conventional methods.

    Original languageEnglish
    Pages (from-to)3531-5
    Number of pages5
    JournalJournal of Clinical Microbiology
    Volume52
    Issue number10
    DOIs
    Publication statusPublished - Oct 2014

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