Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors

Mar Cabeza-Cabrerizo; Janneke van Blijswijk; Stephan Wienert; Daniel Heim; Robert P Jenkins; Probir Chakravarty; Neil Rogers; Bruno Frederico; Sophie Acton; Evelyne Beerling; Jacco van Rheenen; Hans Clevers; Barbara U Schraml; Marc Bajénoff; Michael Gerner; Ronald N Germain; Erik Sahai; Frederick Klauschen; Caetano Reis E Sousa

doi:10.1126/sciimmunol.aaw1941

Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors

Mar Cabeza-Cabrerizo, Janneke van Blijswijk, Stephan Wienert, Daniel Heim, Robert P Jenkins, Probir Chakravarty, Neil Rogers, Bruno Frederico, Sophie Acton, Evelyne Beerling, Jacco van Rheenen, Hans Clevers, Barbara U Schraml, Marc Bajénoff, Michael Gerner, Ronald N Germain, Erik Sahai, Frederick Klauschen, Caetano Reis E Sousa

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

80 Citations (Scopus)

Abstract

Conventional dendritic cells (cDCs) are found in all tissues and play a key role in immune surveillance. They comprise two major subsets, cDC1 and cDC2, both derived from circulating precursors of cDCs (pre-cDCs), which exited the bone marrow. We show that, in the steady-state mouse, pre-cDCs entering tissues proliferate to give rise to differentiated cDCs, which themselves have residual proliferative capacity. We use multicolor fate mapping of cDC progenitors to show that this results in clones of sister cDCs, most of which comprise a single cDC1 or cDC2 subtype, suggestive of pre-cDC commitment. Upon infection, a surge in the influx of pre-cDCs into the affected tissue dilutes clones and increases cDC numbers. Our results indicate that tissue cDCs can be organized in a patchwork of closely positioned sister cells of the same subset whose coexistence is perturbed by local infection, when the bone marrow provides additional pre-cDCs to meet increased tissue demand.

Original language	English
Journal	Science immunology
Volume	4
Issue number	33
DOIs	https://doi.org/10.1126/sciimmunol.aaw1941
Publication status	Published - 01 Mar 2019

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Cabeza-Cabrerizo, M., van Blijswijk, J., Wienert, S., Heim, D., Jenkins, R. P., Chakravarty, P., Rogers, N., Frederico, B., Acton, S., Beerling, E., van Rheenen, J., Clevers, H., Schraml, B. U., Bajénoff, M., Gerner, M., Germain, R. N., Sahai, E., Klauschen, F., & Reis E Sousa, C. (2019). Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors. Science immunology, 4(33). https://doi.org/10.1126/sciimmunol.aaw1941

@article{563c81f2c5b14f25bf8a8dfad9a40aea,

title = "Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors",

abstract = "Conventional dendritic cells (cDCs) are found in all tissues and play a key role in immune surveillance. They comprise two major subsets, cDC1 and cDC2, both derived from circulating precursors of cDCs (pre-cDCs), which exited the bone marrow. We show that, in the steady-state mouse, pre-cDCs entering tissues proliferate to give rise to differentiated cDCs, which themselves have residual proliferative capacity. We use multicolor fate mapping of cDC progenitors to show that this results in clones of sister cDCs, most of which comprise a single cDC1 or cDC2 subtype, suggestive of pre-cDC commitment. Upon infection, a surge in the influx of pre-cDCs into the affected tissue dilutes clones and increases cDC numbers. Our results indicate that tissue cDCs can be organized in a patchwork of closely positioned sister cells of the same subset whose coexistence is perturbed by local infection, when the bone marrow provides additional pre-cDCs to meet increased tissue demand.",

author = "Mar Cabeza-Cabrerizo and {van Blijswijk}, Janneke and Stephan Wienert and Daniel Heim and Jenkins, {Robert P} and Probir Chakravarty and Neil Rogers and Bruno Frederico and Sophie Acton and Evelyne Beerling and {van Rheenen}, Jacco and Hans Clevers and Schraml, {Barbara U} and Marc Baj{\'e}noff and Michael Gerner and Germain, {Ronald N} and Erik Sahai and Frederick Klauschen and {Reis E Sousa}, Caetano",

note = "Copyright {\textcopyright} 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.",

year = "2019",

month = mar,

day = "1",

doi = "10.1126/sciimmunol.aaw1941",

language = "English",

volume = "4",

journal = "Science immunology",

issn = "2470-9468",

publisher = "American Association for the Advancement of Science (AAAS)",

number = "33",

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Cabeza-Cabrerizo, M, van Blijswijk, J, Wienert, S, Heim, D, Jenkins, RP, Chakravarty, P, Rogers, N, Frederico, B, Acton, S, Beerling, E, van Rheenen, J, Clevers, H, Schraml, BU, Bajénoff, M, Gerner, M, Germain, RN, Sahai, E, Klauschen, F & Reis E Sousa, C 2019, 'Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors', Science immunology, vol. 4, no. 33. https://doi.org/10.1126/sciimmunol.aaw1941

TY - JOUR

T1 - Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors

AU - Cabeza-Cabrerizo, Mar

AU - van Blijswijk, Janneke

AU - Wienert, Stephan

AU - Heim, Daniel

AU - Jenkins, Robert P

AU - Chakravarty, Probir

AU - Rogers, Neil

AU - Frederico, Bruno

AU - Acton, Sophie

AU - Beerling, Evelyne

AU - van Rheenen, Jacco

AU - Clevers, Hans

AU - Schraml, Barbara U

AU - Bajénoff, Marc

AU - Gerner, Michael

AU - Germain, Ronald N

AU - Sahai, Erik

AU - Klauschen, Frederick

AU - Reis E Sousa, Caetano

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Conventional dendritic cells (cDCs) are found in all tissues and play a key role in immune surveillance. They comprise two major subsets, cDC1 and cDC2, both derived from circulating precursors of cDCs (pre-cDCs), which exited the bone marrow. We show that, in the steady-state mouse, pre-cDCs entering tissues proliferate to give rise to differentiated cDCs, which themselves have residual proliferative capacity. We use multicolor fate mapping of cDC progenitors to show that this results in clones of sister cDCs, most of which comprise a single cDC1 or cDC2 subtype, suggestive of pre-cDC commitment. Upon infection, a surge in the influx of pre-cDCs into the affected tissue dilutes clones and increases cDC numbers. Our results indicate that tissue cDCs can be organized in a patchwork of closely positioned sister cells of the same subset whose coexistence is perturbed by local infection, when the bone marrow provides additional pre-cDCs to meet increased tissue demand.

AB - Conventional dendritic cells (cDCs) are found in all tissues and play a key role in immune surveillance. They comprise two major subsets, cDC1 and cDC2, both derived from circulating precursors of cDCs (pre-cDCs), which exited the bone marrow. We show that, in the steady-state mouse, pre-cDCs entering tissues proliferate to give rise to differentiated cDCs, which themselves have residual proliferative capacity. We use multicolor fate mapping of cDC progenitors to show that this results in clones of sister cDCs, most of which comprise a single cDC1 or cDC2 subtype, suggestive of pre-cDC commitment. Upon infection, a surge in the influx of pre-cDCs into the affected tissue dilutes clones and increases cDC numbers. Our results indicate that tissue cDCs can be organized in a patchwork of closely positioned sister cells of the same subset whose coexistence is perturbed by local infection, when the bone marrow provides additional pre-cDCs to meet increased tissue demand.

U2 - 10.1126/sciimmunol.aaw1941

DO - 10.1126/sciimmunol.aaw1941

M3 - Article

C2 - 30824528

SN - 2470-9468

VL - 4

JO - Science immunology

JF - Science immunology

IS - 33

ER -

Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors

Abstract

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