Abstract
We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is broadly applicable to profiling transcriptionally distinct cellular states without requiring antibodies or transgenic fluorescent proteins.
Original language | English |
---|---|
Pages (from-to) | 549-51 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 11 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2014 |
Keywords
- Alleles
- Animals
- Cell Culture Techniques
- Cellular Reprogramming
- Doxycycline
- Embryonic Stem Cells
- Fibroblasts
- Flow Cytometry
- Gene Expression Profiling
- Genome-Wide Association Study
- Green Fluorescent Proteins
- In Situ Hybridization, Fluorescence
- Induced Pluripotent Stem Cells
- Mice
- NIH 3T3 Cells
- Oligonucleotide Array Sequence Analysis
- Polymerase Chain Reaction
- RNA
- RNA, Messenger
- Transcription, Genetic
- Transgenes