Unique Phylogenetic Distributions of the Ska and Dam1 Complexes Support Functional Analogy and Suggest Multiple Parallel Displacements of Ska by Dam1

Jolien J E van Hooff, Berend Snel, Geert J P L Kops

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Faithful chromosome segregation relies on kinetochores, the large protein complexes that connect chromatin to spindle microtubules. Although human and yeast kinetochores are largely homologous, they track microtubules with the unrelated protein complexes Ska (Ska-C, human) and Dam1 (Dam1-C, yeast). We here uncovered that Ska-C and Dam1-C are both widespread among eukaryotes, but in an exceptionally inverse manner, supporting their functional analogy. Within the complexes, all Ska-C and various Dam1-C subunits are ancient paralogs, showing that gene duplication shaped these complexes. We examined various evolutionary scenarios to explain the nearly mutually exclusive patterns of Ska-C and Dam1-C in present-day species. We propose that Ska-C was present in the last eukaryotic common ancestor, that subsequently Dam1-C displaced Ska-C in an early fungus and was horizontally transferred to diverse non-fungal lineages, displacing Ska-C in these lineages too.

Original languageEnglish
Pages (from-to)1295-1303
Number of pages9
JournalGenome Biology and Evolution
Volume9
Issue number5
DOIs
Publication statusPublished - 01 May 2017

Keywords

  • Chromosomal Proteins, Non-Histone
  • Evolution, Molecular
  • Gene Duplication
  • Gene Transfer, Horizontal
  • Humans
  • Kinetochores
  • Microtubule-Associated Proteins
  • Microtubules
  • Phylogeny
  • Saccharomyces cerevisiae
  • Journal Article

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