Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon

Danielle Machado Pagani; Stefânia P.R. Ventura; Duong Vu; Thairine Mendes-Pereira; Luiz Marcelo Ribeiro Tomé; Daniel Santana de Carvalho; Diogo Henrique Costa-Rezende; Rodrigo Bentes Kato; Glen Jasper Yupanqui García; József Geml; Vincent Robert; Ng Haig They; Bertram Brenig; Vasco Azevedo; Maria Lúcia Scroferneker; Patricia Valente; Aristóteles Góes-Neto

doi:10.3390/jof9090890

Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon

Danielle Machado Pagani, Stefânia P.R. Ventura, Duong Vu, Thairine Mendes-Pereira, Luiz Marcelo Ribeiro Tomé, Daniel Santana de Carvalho, Diogo Henrique Costa-Rezende, Rodrigo Bentes Kato, Glen Jasper Yupanqui García, József Geml, Vincent Robert, Ng Haig They, Bertram Brenig, Vasco Azevedo, Maria Lúcia Scroferneker, Patricia Valente, Aristóteles Góes-Neto^*

^*Corresponding author for this work

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

Studies of fungal communities through amplicon metagenomics in aquatic environments, particularly in freshwater ecosystems, are still relatively recent. Unfortunately, many of these water bodies are facing growing threats from human expansion, such as effluent discharge from various human activities. As a result, these effluents have the potential to significantly alter the characteristics of water bodies and, subsequently, impact the diversity of their resident microorganisms. In this context, our objective was to investigate whether the fungal community structure varies according to the presence of different anthropic disturbances. We expect (i) the diversity of fungi will be greater and (ii) more specific unique operational taxonomic units (OTUs) related to each ecotonal system will be found compared to other sites of a lagoon. The study was conducted in the Tramandaí Lagoon (subtropical southern Brazil) at four distinct sampling points (estuary, middle of the lagoon, crop field area, and near a residential area where the Tramandaí River flows into the lagoon). As expected, the estuary and residential zones, which are ecotones, exhibited greater fungal diversity and more specific OTUs compared to the middle of the lagoon and crop field area. Moreover, a substantial proportion of fungal taxa could not be identified at the genus level, with many only classified at the phylum level, indicating potential new lineages. These findings underscore our limited understanding of the subtropical freshwater mycobiota.

Original language	English
Article number	890
Journal	Journal of Fungi
Volume	9
Issue number	9
DOIs	https://doi.org/10.3390/jof9090890
Publication status	Published - Sept 2023

Keywords

amplicon metagenomics
freshwater
fungi

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10.3390/jof9090890

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Pagani, D. M., Ventura, S. P. R., Vu, D., Mendes-Pereira, T., Ribeiro Tomé, L. M., Carvalho, D. S. D., Costa-Rezende, D. H., Kato, R. B., García, G. J. Y., Geml, J., Robert, V., They, N. H., Brenig, B., Azevedo, V., Scroferneker, M. L., Valente, P., & Góes-Neto, A. (2023). Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon. Journal of Fungi, 9(9), Article 890. https://doi.org/10.3390/jof9090890

@article{6470b7ea66b54bdaadc5682c60e1b0f8,

title = "Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon",

abstract = "Studies of fungal communities through amplicon metagenomics in aquatic environments, particularly in freshwater ecosystems, are still relatively recent. Unfortunately, many of these water bodies are facing growing threats from human expansion, such as effluent discharge from various human activities. As a result, these effluents have the potential to significantly alter the characteristics of water bodies and, subsequently, impact the diversity of their resident microorganisms. In this context, our objective was to investigate whether the fungal community structure varies according to the presence of different anthropic disturbances. We expect (i) the diversity of fungi will be greater and (ii) more specific unique operational taxonomic units (OTUs) related to each ecotonal system will be found compared to other sites of a lagoon. The study was conducted in the Tramanda{\'i} Lagoon (subtropical southern Brazil) at four distinct sampling points (estuary, middle of the lagoon, crop field area, and near a residential area where the Tramanda{\'i} River flows into the lagoon). As expected, the estuary and residential zones, which are ecotones, exhibited greater fungal diversity and more specific OTUs compared to the middle of the lagoon and crop field area. Moreover, a substantial proportion of fungal taxa could not be identified at the genus level, with many only classified at the phylum level, indicating potential new lineages. These findings underscore our limited understanding of the subtropical freshwater mycobiota.",

keywords = "amplicon metagenomics, freshwater, fungi",

author = "Pagani, {Danielle Machado} and Ventura, {Stef{\^a}nia P.R.} and Duong Vu and Thairine Mendes-Pereira and {Ribeiro Tom{\'e}}, {Luiz Marcelo} and Carvalho, {Daniel Santana de} and Costa-Rezende, {Diogo Henrique} and Kato, {Rodrigo Bentes} and Garc{\'i}a, {Glen Jasper Yupanqui} and J{\'o}zsef Geml and Vincent Robert and They, {Ng Haig} and Bertram Brenig and Vasco Azevedo and Scroferneker, {Maria L{\'u}cia} and Patricia Valente and Arist{\'o}teles G{\'o}es-Neto",

note = "Funding Information: This study was funded by the Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) and Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES). S.P.R.V. was funded by Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de Minas Gerais (FAPEMIG) in the grant Process APQ-01411-22; A.G.-N. was funded by CNPq in the grant process 308880/2022-6. Funding Information: We would like to thank the Graduate Programs of Microbiology and Bioinformatics of the Universidade Federal de Minas Gerais (UFMG), the Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) and Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES). Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = sep,

doi = "10.3390/jof9090890",

language = "English",

volume = "9",

journal = "Journal of Fungi",

issn = "2309-608X",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "9",

}

Pagani, DM, Ventura, SPR, Vu, D, Mendes-Pereira, T, Ribeiro Tomé, LM, Carvalho, DSD, Costa-Rezende, DH, Kato, RB, García, GJY, Geml, J, Robert, V, They, NH, Brenig, B, Azevedo, V, Scroferneker, ML, Valente, P & Góes-Neto, A 2023, 'Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon', Journal of Fungi, vol. 9, no. 9, 890. https://doi.org/10.3390/jof9090890

TY - JOUR

T1 - Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon

AU - Pagani, Danielle Machado

AU - Ventura, Stefânia P.R.

AU - Vu, Duong

AU - Mendes-Pereira, Thairine

AU - Ribeiro Tomé, Luiz Marcelo

AU - Carvalho, Daniel Santana de

AU - Costa-Rezende, Diogo Henrique

AU - Kato, Rodrigo Bentes

AU - García, Glen Jasper Yupanqui

AU - Geml, József

AU - Robert, Vincent

AU - They, Ng Haig

AU - Brenig, Bertram

AU - Azevedo, Vasco

AU - Scroferneker, Maria Lúcia

AU - Valente, Patricia

AU - Góes-Neto, Aristóteles

N1 - Funding Information: This study was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). S.P.R.V. was funded by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) in the grant Process APQ-01411-22; A.G.-N. was funded by CNPq in the grant process 308880/2022-6. Funding Information: We would like to thank the Graduate Programs of Microbiology and Bioinformatics of the Universidade Federal de Minas Gerais (UFMG), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Publisher Copyright: © 2023 by the authors.

PY - 2023/9

Y1 - 2023/9

N2 - Studies of fungal communities through amplicon metagenomics in aquatic environments, particularly in freshwater ecosystems, are still relatively recent. Unfortunately, many of these water bodies are facing growing threats from human expansion, such as effluent discharge from various human activities. As a result, these effluents have the potential to significantly alter the characteristics of water bodies and, subsequently, impact the diversity of their resident microorganisms. In this context, our objective was to investigate whether the fungal community structure varies according to the presence of different anthropic disturbances. We expect (i) the diversity of fungi will be greater and (ii) more specific unique operational taxonomic units (OTUs) related to each ecotonal system will be found compared to other sites of a lagoon. The study was conducted in the Tramandaí Lagoon (subtropical southern Brazil) at four distinct sampling points (estuary, middle of the lagoon, crop field area, and near a residential area where the Tramandaí River flows into the lagoon). As expected, the estuary and residential zones, which are ecotones, exhibited greater fungal diversity and more specific OTUs compared to the middle of the lagoon and crop field area. Moreover, a substantial proportion of fungal taxa could not be identified at the genus level, with many only classified at the phylum level, indicating potential new lineages. These findings underscore our limited understanding of the subtropical freshwater mycobiota.

AB - Studies of fungal communities through amplicon metagenomics in aquatic environments, particularly in freshwater ecosystems, are still relatively recent. Unfortunately, many of these water bodies are facing growing threats from human expansion, such as effluent discharge from various human activities. As a result, these effluents have the potential to significantly alter the characteristics of water bodies and, subsequently, impact the diversity of their resident microorganisms. In this context, our objective was to investigate whether the fungal community structure varies according to the presence of different anthropic disturbances. We expect (i) the diversity of fungi will be greater and (ii) more specific unique operational taxonomic units (OTUs) related to each ecotonal system will be found compared to other sites of a lagoon. The study was conducted in the Tramandaí Lagoon (subtropical southern Brazil) at four distinct sampling points (estuary, middle of the lagoon, crop field area, and near a residential area where the Tramandaí River flows into the lagoon). As expected, the estuary and residential zones, which are ecotones, exhibited greater fungal diversity and more specific OTUs compared to the middle of the lagoon and crop field area. Moreover, a substantial proportion of fungal taxa could not be identified at the genus level, with many only classified at the phylum level, indicating potential new lineages. These findings underscore our limited understanding of the subtropical freshwater mycobiota.

KW - amplicon metagenomics

KW - freshwater

KW - fungi

UR - http://www.scopus.com/inward/record.url?scp=85172761440&partnerID=8YFLogxK

U2 - 10.3390/jof9090890

DO - 10.3390/jof9090890

M3 - Article

AN - SCOPUS:85172761440

SN - 2309-608X

VL - 9

JO - Journal of Fungi

JF - Journal of Fungi

IS - 9

M1 - 890

ER -

Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon

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Keywords

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