Unveiling the fungal mycobiota present throughout the cork stopper manufacturing process

TY - JOUR

T1 - Unveiling the fungal mycobiota present throughout the cork stopper manufacturing process

AU - Barreto, M.C.

AU - Houbraken, J.

AU - Samson, R.A.

AU - Brito, D.

AU - Gadanho, M.

AU - San Romão, M.V.

N1 - Reporting year: 2012

PY - 2012

Y1 - 2012

N2 - A particular fungal population is present in the main stages of the manufacturing process of cork discs. Its diversity was studied using both dependent (isolation) and independent culture methods (denaturing gel gradient electrophoresis and cloning of the ITS1-5.8S-ITS2 region). The mycobiota in the samples taken in the stages before and after the first boiling seems to be distinct from the population in the subsequent manufacturing stages. Most isolated fungi belong to the genera Penicillium, Eurotium and Cladosporium. The presence of uncultivable fungi, Ascomycota and endophytes in raw cork was confirmed by sequencing. The samples taken after the first boiling contained uncultivable fungi, but in a few samples some isolated fungi were also detected. The main taxa present in the following stages were Chrysonilia sitophila, Penicillium glabrum and Penicillium spp. All applied techniques had complementary outcomes. The main factors driving the shift in cork fungal colonization seem to be the high levels of humidity and temperature to which the slabs are subjected during the boiling process. © 2012 Federation of European Microbiological Societies.

AB - A particular fungal population is present in the main stages of the manufacturing process of cork discs. Its diversity was studied using both dependent (isolation) and independent culture methods (denaturing gel gradient electrophoresis and cloning of the ITS1-5.8S-ITS2 region). The mycobiota in the samples taken in the stages before and after the first boiling seems to be distinct from the population in the subsequent manufacturing stages. Most isolated fungi belong to the genera Penicillium, Eurotium and Cladosporium. The presence of uncultivable fungi, Ascomycota and endophytes in raw cork was confirmed by sequencing. The samples taken after the first boiling contained uncultivable fungi, but in a few samples some isolated fungi were also detected. The main taxa present in the following stages were Chrysonilia sitophila, Penicillium glabrum and Penicillium spp. All applied techniques had complementary outcomes. The main factors driving the shift in cork fungal colonization seem to be the high levels of humidity and temperature to which the slabs are subjected during the boiling process. © 2012 Federation of European Microbiological Societies.

U2 - 10.1111/j.1574-6941.2012.01419.x

DO - 10.1111/j.1574-6941.2012.01419.x

M3 - Article

SN - 0168-6496

VL - 82

SP - 202

EP - 214

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 1

ER -