• S. te Lintel Hekkert
  • M.J. Staal
  • R.H.M. Nabben
  • H. Zuckermann
  • S. Persijn
  • L.J. Stal
  • L.A.C.J. Voesenek
  • F.J.M. Harren
  • J. Reuss
  • D.H. Parker
The use of infrared laser based photo-acoustic trace gas detection equipment in biological research is discussed on the basis bf two examples. A CO2 laser based photo-acoustic trace gas detection system is employed to follow the time-dependent pattern of the nitrogen fixation process by the cyanobacteria Nodularia Spumigena on a one-minute time scale. Due to the high sensitivity of the detection system for ethylene (detection limit 6 part per trillion; 6.10(12)), the fixation process can be followed on-line in a flow-through system. Following a 50 h dark incubation period, the bacteria show nitrogen fixation only after a certain illumination period, indicating lack of carbohydrates needed to start the nitrogen fixation. [KEYWORDS: CO-LASER; PLANTS; ACETALDEHYDE; SPECTROSCOPY; ADAPTATIONS; CO2-LASER; RESPONSES; ETHYLENE; ECOLOGY; ANOXIA]
Original languageEnglish
Pages (from-to)157-175
JournalInstrumentation Science and Technology
Issue number2-3
StatePublished - 1998

ID: 386415