• I. Geudens
  • K. Hermans
  • I. Segura
  • C. Ruiz de Almodovar
  • F. De Smet
  • W. Vandevelde
  • A. Siekmann
  • F. Claes
  • J. Moore
  • A.S. Pistocchi
  • S. Loges
  • M. Mazzone
  • G. Mariggi
  • F. Bruyere
  • F. Cotelli
  • D. Kerjaschki
  • A. Noel
  • J.M. Foidart
  • H. Gerhardt
  • A. Ny
  • T. Langenberg
  • N. Lawson
  • H.J. Duckers
  • P. Carmeliet
  • M. Dewerchin
OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
Original languageEnglish
Pages (from-to)1695-1702
JournalArtheriosclerosis, Thrombosis and Vascular Biology
Issue number9
StatePublished - 2010

ID: 245347