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We performed a 15N-labeling study to investigate nitrogen incorporation and retention by the benthic microbial community (bacteria and benthic microalgae) and fauna in the intertidal sediment of the subtropical Australian Brunswick Estuary. The main experiment involved an in situ 15N pulse–chase experiment. After injection of 15NH4+ into the sediment, 15N was traced into bulk sediment, total hydrolyzable amino acids (THAAs, representing bulk proteinaceous biomass), the bacterial biomarker D-alanine, and fauna over a 30- d period. Additional experiments included short-term (24 h) incubations of sediment cores injected with different 15N-labeled substrates (NH4+, NO3-, urea, and an amino acid mixture) and sediment core incubations for analysis of benthic fluxes of O2, dissolved inorganic carbon, NH4+, NOx-, dissolved organic nitrogen, and N2. 15N was rapidly incorporated and strongly retained in microbial biomass (THAAs) during the 30-d period in situ, indicating efficient recycling of 15N by the benthic microbial community. Analysis of 15N in D-alanine revealed a major bacterial contribution (50–100%) to total microbial 15N incorporation and retention. 15N was also incorporated into fauna via grazing on 15N-labeled microbial biomass, but this was a negligible fraction (
Original languageEnglish
Pages (from-to)1930-1942
JournalLimnology and Oceanography
Issue number5
StatePublished - 2007

ID: 190415