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  • I.A. Ivanova
  • J.R. Stephen
  • Y.J. Chang
  • J. Bruggemann
  • P.E. Long
  • J.P. McKinley
  • G.A. Kowalchuk
  • D.C. White
  • S.J. MacNaughton
In this study, we investigated the size and structure of autotrophic ammonia oxidizer (AAO) communities in the groundwater of a contamination plume originating from a mill- tailings disposal site. The site has high levels of dissolved N from anthropogenic sources, and exhibited wide variations in the concentrations of NO3- and NH3 + NH4+. Community structures were examined by PCR-DGGE targeting 16S rDNA with band excision and sequence analysis, and by analysis of amoA fragment clone libraries. AAO population sizes were estimated by competitive PCR targeting the gene amoA, and correlated significantly with nitrate concentration. Most samples revealed novel diversity in AAO 16S rDNA and amoA gene sequences. Both 16S rDNA and amoA analyses suggested that all samples were dominated by Nitrosomonas sp., Nitrosospira sp. being detected in only 3 of 15 samples. This study indicated numerical dominance of Nitrosomonas over Nitrosospira in groundwater, and suggests that groundwater ammonia oxidizers are more similar to those dominating freshwater sediments than bulk soil. [KEYWORDS: Nitrosomonas; Nitrosospira; nitrification; groundwater; amoA;16S Gradient gel-electrophoresis; ribosomal-rna genes; nitrosospira sp; nitrosomonas-europaea; molecular analysis; monooxygenase; sequences; soil; populations; diversity]
Original languageEnglish
Pages (from-to)1012-1020
JournalCanadian Journal of Microbiology
Volume46
Issue number11
DOI
StatePublished - 2000

ID: 367591