• W. Sprenger
  • A. Dijkstra
  • G. Zwart
  • M.P. Van Agterveld
  • P.C.M. Van Noort
  • J.R. Parsons
The effect of competition for macroelements with bacteria from ditch water on the parathion-hydrolyzing Flavobacterium sp. ATCC 27551 (FB) was investigated within mixed continuous cultures under carbon-, nitrate- or phosphate-limited conditions. The high initial rate of parathion hydrolysis decreased rapidly in all cultures due to the loss of strain FB. Addition of 2-isopropyl-6-methyl-4-pyrimidinol (a selective source of carbon, nitrogen and energy for FB) to one nitrate- and carbon-limited chemostat caused a 20-fold increase in parathion-hydrolyzing activity compared to unamended control cultures and retention of FB. The presence of the parathion hydrolase-encoding gene could be demonstrated by a newly developed PCR detection method in all FB cultures during most of the cultivation period. These results suggest that competition effects cause the pesticide-degrading capacity of microbial communities depending on their frequency of exposure to the pesticide compounds. [KEYWORDS: Organophosphorus; Pesticide; Biodegradation; Nutrient; Competition; Chemostat]
Original languageEnglish
JournalFEMS Microbiology Ecology
Journal publication date2003

ID: 187573