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DOI

Net nitrification in intact soil cores and the community of ammonia-oxidising bacteria were studied in acid Norway spruce (Picea abies (L.) Karst) and sessile oak (Quercus petraea (Matt. Lieb.)) soils (Haute Ardenne, east Belgium) 18 months after treatment with 5tha1 dolomite lime. Liming caused a significant increase in nitrification in the upper soil layers (0.15m) of both stands. DGGE (denaturing gradient gel electrophoresis) profiling after -subclass ammonia oxidiser-specific polymerase chain reaction (PCR), combined with hybridisation and sequencing of excised DGGE bands revealed a dominance of Nitrosomonas europaea-like sequences, independent of soil horizon, tree species and lime treatment. A minority Nitrosospira-like population was detected, which showed affinity to nitrosospiras previously detected in acid soil. These results contrast with several reports suggesting a dominance of Nitrosospira-like organisms among ammonia oxidiser communities in acid soils. [KEYWORDS: 16S rDNA; DGGE; Picea abies; Quercus petraea; Nitrification; Dolomite lime]
Original languageEnglish
Pages (from-to)1047-1050
JournalSoil Biology & Biochemistry
Volume34
Issue number7
DOI
StatePublished - 2002

ID: 133870