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Denaturant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual ‘double bands’ that can be encountered in several applications. [KEYWORDS: DGGE ; Double bands ; Artifacts]
Original languageEnglish
Pages (from-to)279-281
JournalJournal of Microbiological Methods
Issue number2
StatePublished - 2004

ID: 88995