Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice

Claudia Kathe, Frédéric Michoud, Philipp Schönle, Andreas Rowald, Noé Brun, Jimmy Ravier, Ivan Furfaro, Valentina Paggi, Kyungjin Kim, Sadaf Soloukey, Leonie Asboth, Thomas H Hutson, Ileana Jelescu, Antoine Philippides, Noaf Alwahab, Jérôme Gandar, Daniel Huber, Chris I De Zeeuw, Quentin Barraud, Qiuting HuangStéphanie P Lacour, Grégoire Courtine

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Optoelectronic systems can exert precise control over targeted neurons and pathways throughout the brain in untethered animals, but similar technologies for the spinal cord are not well established. In the present study, we describe a system for ultrafast, wireless, closed-loop manipulation of targeted neurons and pathways across the entire dorsoventral spinal cord in untethered mice. We developed a soft stretchable carrier, integrating microscale light-emitting diodes (micro-LEDs), that conforms to the dura mater of the spinal cord. A coating of silicone-phosphor matrix over the micro-LEDs provides mechanical protection and light conversion for compatibility with a large library of opsins. A lightweight, head-mounted, wireless platform powers the micro-LEDs and performs low-latency, on-chip processing of sensed physiological signals to control photostimulation in a closed loop. We use the device to reveal the role of various neuronal subtypes, sensory pathways and supraspinal projections in the control of locomotion in healthy and spinal-cord injured mice.

Original languageEnglish
JournalNature Biotechnology
DOIs
Publication statusE-pub ahead of print - 27 Sep 2021

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