TY - JOUR
T1 - Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice
AU - Kathe, Claudia
AU - Michoud, Frédéric
AU - Schönle, Philipp
AU - Rowald, Andreas
AU - Brun, Noé
AU - Ravier, Jimmy
AU - Furfaro, Ivan
AU - Paggi, Valentina
AU - Kim, Kyungjin
AU - Soloukey, Sadaf
AU - Asboth, Leonie
AU - Hutson, Thomas H
AU - Jelescu, Ileana
AU - Philippides, Antoine
AU - Alwahab, Noaf
AU - Gandar, Jérôme
AU - Huber, Daniel
AU - De Zeeuw, Chris I
AU - Barraud, Quentin
AU - Huang, Qiuting
AU - Lacour, Stéphanie P
AU - Courtine, Grégoire
N1 - © 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2022/1
Y1 - 2022/1
N2 - Optoelectronic systems can exert precise control over targeted neurons and pathways throughout the brain in untethered animals, but similar technologies for the spinal cord are not well established. In the present study, we describe a system for ultrafast, wireless, closed-loop manipulation of targeted neurons and pathways across the entire dorsoventral spinal cord in untethered mice. We developed a soft stretchable carrier, integrating microscale light-emitting diodes (micro-LEDs), that conforms to the dura mater of the spinal cord. A coating of silicone-phosphor matrix over the micro-LEDs provides mechanical protection and light conversion for compatibility with a large library of opsins. A lightweight, head-mounted, wireless platform powers the micro-LEDs and performs low-latency, on-chip processing of sensed physiological signals to control photostimulation in a closed loop. We use the device to reveal the role of various neuronal subtypes, sensory pathways and supraspinal projections in the control of locomotion in healthy and spinal-cord injured mice.
AB - Optoelectronic systems can exert precise control over targeted neurons and pathways throughout the brain in untethered animals, but similar technologies for the spinal cord are not well established. In the present study, we describe a system for ultrafast, wireless, closed-loop manipulation of targeted neurons and pathways across the entire dorsoventral spinal cord in untethered mice. We developed a soft stretchable carrier, integrating microscale light-emitting diodes (micro-LEDs), that conforms to the dura mater of the spinal cord. A coating of silicone-phosphor matrix over the micro-LEDs provides mechanical protection and light conversion for compatibility with a large library of opsins. A lightweight, head-mounted, wireless platform powers the micro-LEDs and performs low-latency, on-chip processing of sensed physiological signals to control photostimulation in a closed loop. We use the device to reveal the role of various neuronal subtypes, sensory pathways and supraspinal projections in the control of locomotion in healthy and spinal-cord injured mice.
U2 - 10.1038/s41587-021-01019-x
DO - 10.1038/s41587-021-01019-x
M3 - Article
C2 - 34580478
SN - 1087-0156
VL - 40
SP - 198
EP - 208
JO - Nature Biotechnology
JF - Nature Biotechnology
ER -