Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

Jiali Meng; Tania Chroumpi; Miia R Mäkelä; Ronald P de Vries

doi:10.1016/j.biortech.2021.126199

Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

Jiali Meng, Tania Chroumpi, Miia R Mäkelä, Ronald P de Vries

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

23 Citations (Scopus)

Abstract

Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.

Original language	English
Pages (from-to)	126199
Journal	Bioresource Technology
Volume	344
Issue number	Pt A
DOIs	https://doi.org/10.1016/j.biortech.2021.126199
Publication status	Published - Jan 2022

Keywords

Aspergillus niger/genetics
Biomass
Fermentation
Metabolic Engineering
Xylitol
Xylose

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10.1016/j.biortech.2021.126199

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title = "Xylitol production from plant biomass by Aspergillus niger through metabolic engineering",

abstract = "Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.",

keywords = "Aspergillus niger/genetics, Biomass, Fermentation, Metabolic Engineering, Xylitol, Xylose",

author = "Jiali Meng and Tania Chroumpi and M{\"a}kel{\"a}, {Miia R} and {de Vries}, {Ronald P}",

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doi = "10.1016/j.biortech.2021.126199",

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journal = "Bioresource Technology",

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T1 - Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

AU - Meng, Jiali

AU - Chroumpi, Tania

AU - Mäkelä, Miia R

AU - de Vries, Ronald P

PY - 2022/1

Y1 - 2022/1

N2 - Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.

AB - Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.

KW - Aspergillus niger/genetics

KW - Biomass

KW - Fermentation

KW - Metabolic Engineering

KW - Xylitol

KW - Xylose

U2 - 10.1016/j.biortech.2021.126199

DO - 10.1016/j.biortech.2021.126199

M3 - Article

C2 - 34710597

SN - 0960-8524

VL - 344

SP - 126199

JO - Bioresource Technology

JF - Bioresource Technology

IS - Pt A

ER -

Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

Abstract

Keywords

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