Zebrafish as a model to study PTPs during development

Jeroen Paardekooper Overman, Jeroen den Hertog

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Protein-tyrosine phosphatases (PTPs) have important roles in signaling, but relatively little is known about their function in vivo. We are using the zebrafish as a model to study the function of PTPs at the organismal, cellular and molecular level. The zebrafish is an excellent experimental model for the analysis of gene function. We have developed methods to quantitatively study effects of PTP knockdown or expression of (mutant) PTPs, particularly with respect to gastrulation cell movements. Moreover, we have studied the phosphoproteome of zebrafish embryos. In this review, we will discuss methods to manipulate the zebrafish genome and techniques that we have developed to assess developmental defects during gastrulation and to assess differences in the phosphoproteome.

Original languageEnglish
Pages (from-to)247-53
Number of pages7
JournalMethods
Volume65
Issue number2
DOIs
Publication statusPublished - 15 Jan 2014

Keywords

  • Animals
  • Gene Expression Regulation, Developmental
  • Models, Animal
  • Protein Tyrosine Phosphatases
  • Proteomics
  • Zebrafish

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