Zebrafish as a model to study PTPs during development

Jeroen Paardekooper Overman; Jeroen den Hertog

doi:10.1016/j.ymeth.2013.08.018

Zebrafish as a model to study PTPs during development

Jeroen Paardekooper Overman, Jeroen den Hertog

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

4 Citations (Scopus)

Abstract

Protein-tyrosine phosphatases (PTPs) have important roles in signaling, but relatively little is known about their function in vivo. We are using the zebrafish as a model to study the function of PTPs at the organismal, cellular and molecular level. The zebrafish is an excellent experimental model for the analysis of gene function. We have developed methods to quantitatively study effects of PTP knockdown or expression of (mutant) PTPs, particularly with respect to gastrulation cell movements. Moreover, we have studied the phosphoproteome of zebrafish embryos. In this review, we will discuss methods to manipulate the zebrafish genome and techniques that we have developed to assess developmental defects during gastrulation and to assess differences in the phosphoproteome.

Original language	English
Pages (from-to)	247-53
Number of pages	7
Journal	Methods
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2013.08.018
Publication status	Published - 15 Jan 2014

Keywords

Animals
Gene Expression Regulation, Developmental
Models, Animal
Protein Tyrosine Phosphatases
Proteomics
Zebrafish

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10.1016/j.ymeth.2013.08.018

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title = "Zebrafish as a model to study PTPs during development",

abstract = "Protein-tyrosine phosphatases (PTPs) have important roles in signaling, but relatively little is known about their function in vivo. We are using the zebrafish as a model to study the function of PTPs at the organismal, cellular and molecular level. The zebrafish is an excellent experimental model for the analysis of gene function. We have developed methods to quantitatively study effects of PTP knockdown or expression of (mutant) PTPs, particularly with respect to gastrulation cell movements. Moreover, we have studied the phosphoproteome of zebrafish embryos. In this review, we will discuss methods to manipulate the zebrafish genome and techniques that we have developed to assess developmental defects during gastrulation and to assess differences in the phosphoproteome.",

keywords = "Animals, Gene Expression Regulation, Developmental, Models, Animal, Protein Tyrosine Phosphatases, Proteomics, Zebrafish",

author = "{Paardekooper Overman}, Jeroen and {den Hertog}, Jeroen",

note = "Copyright {\textcopyright} 2014. Published by Elsevier Inc.",

year = "2014",

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doi = "10.1016/j.ymeth.2013.08.018",

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T1 - Zebrafish as a model to study PTPs during development

AU - Paardekooper Overman, Jeroen

AU - den Hertog, Jeroen

PY - 2014/1/15

Y1 - 2014/1/15

N2 - Protein-tyrosine phosphatases (PTPs) have important roles in signaling, but relatively little is known about their function in vivo. We are using the zebrafish as a model to study the function of PTPs at the organismal, cellular and molecular level. The zebrafish is an excellent experimental model for the analysis of gene function. We have developed methods to quantitatively study effects of PTP knockdown or expression of (mutant) PTPs, particularly with respect to gastrulation cell movements. Moreover, we have studied the phosphoproteome of zebrafish embryos. In this review, we will discuss methods to manipulate the zebrafish genome and techniques that we have developed to assess developmental defects during gastrulation and to assess differences in the phosphoproteome.

AB - Protein-tyrosine phosphatases (PTPs) have important roles in signaling, but relatively little is known about their function in vivo. We are using the zebrafish as a model to study the function of PTPs at the organismal, cellular and molecular level. The zebrafish is an excellent experimental model for the analysis of gene function. We have developed methods to quantitatively study effects of PTP knockdown or expression of (mutant) PTPs, particularly with respect to gastrulation cell movements. Moreover, we have studied the phosphoproteome of zebrafish embryos. In this review, we will discuss methods to manipulate the zebrafish genome and techniques that we have developed to assess developmental defects during gastrulation and to assess differences in the phosphoproteome.

KW - Animals

KW - Gene Expression Regulation, Developmental

KW - Models, Animal

KW - Protein Tyrosine Phosphatases

KW - Proteomics

KW - Zebrafish

U2 - 10.1016/j.ymeth.2013.08.018

DO - 10.1016/j.ymeth.2013.08.018

M3 - Article

C2 - 23974070

SN - 1046-2023

VL - 65

SP - 247

EP - 253

JO - Methods

JF - Methods

IS - 2

ER -

Zebrafish as a model to study PTPs during development

Abstract

Keywords

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