Several biodiversity studies have shown that soil is a great reservoir of organisms including fungi. Especially, culture- independent approaches using methods such high throughput sequencing (HTS) and metabarcoding have revealed a considerably increase in the expected number of species and at the same time are giving new insights in fungal ecology and proven as a powerful tool for monitoring studies. However, culture-dependent methods remain valuable as source for obtaining and preserving that biodiversity for further studies that can lead to a better knowledge of the role they play in the ecosystems or as a basis for biotechnological applications. By having a pure culture, particularly, in taxonomic studies where a species level identification is necessary, allow us to implement different morphological and molecular studies and in addition many of those unknown taxa can be described.