DNA isolation and purification was done as mentioned in Sepers et al. (2021). The RRBS libraries were prepared with the Ovation RRBS Methyl-Seq System 1-16 kit from NuGEN. The final amplified samples were sequenced in 2018 on an Illumina HiSeq 4000 (100 bp from single-end reads). Construction of the libraries and bisulfite sequencing was done by the Roy J. Carver Biotechnology Centre (University of Illinois at Urbana-Champaign, United States).
Gawehns, F. (Maker), Postuma, M. (Maker), Van Antro, M. (Maker), Nunn, A. (Maker), Sepers, B. (Maker), Fatma, S. (Maker), Van Gurp, T. (Maker), Wagemaker, N. C. A. M. (Maker), Mateman, A. C. (Maker), Milanovic-Ivanovic, S. (Maker), Grosse, I. (Maker), van Oers, K. (Maker), Vergeer, P. (Maker), Verhoeven, K. (Maker) (2022). SRX10989860: RRBS individual Parus major. NCBI.