Samenvatting
The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that force-activated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.
Originele taal-2 | Engels |
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Pagina's (van-tot) | 1779-91 |
Aantal pagina's | 13 |
Tijdschrift | Journal of Cell Science |
Volume | 127 |
Nummer van het tijdschrift | Pt 8 |
DOI's | |
Status | Gepubliceerd - 15 apr. 2014 |