3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

Susanne C van den Brink; Alexander van Oudenaarden

doi:10.1016/j.tcb.2021.06.007

3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

Susanne C van den Brink, Alexander van Oudenaarden

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Boek/film/artikelrecensie › Wetenschappelijk

63 Citaten (Scopus)

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3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high-throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.

Originele taal-2	Engels
Pagina's (van-tot)	747-759
Aantal pagina's	13
Tijdschrift	Trends in Cell Biology
Volume	31
Nummer van het tijdschrift	9
DOI's	https://doi.org/10.1016/j.tcb.2021.06.007
Status	Gepubliceerd - sep. 2021

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title = "3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation",

abstract = "3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high-throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.",

keywords = "Animals, Embryonic Development, Embryonic Stem Cells, Gastrula, Gastrulation, Models, Biological",

author = "{van den Brink}, {Susanne C} and {van Oudenaarden}, Alexander",

year = "2021",

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doi = "10.1016/j.tcb.2021.06.007",

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AU - van Oudenaarden, Alexander

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AB - 3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-stage embryos, have emerged as a powerful tool to study the early stages of mammalian post-implantation development in vitro. Owing to their tractable nature and the relative ease by which they can be generated in large numbers, 3D gastruloids provide an unparalleled opportunity to study normal and pathological embryogenesis from a bottom-up perspective and in a high-throughput manner. Here, we review how gastruloid models can be exploited to deepen our understanding of mammalian development. In addition, we discuss current limitations, potential clinical applications, and ethical implications of this emerging model system.

KW - Animals

KW - Embryonic Development

KW - Embryonic Stem Cells

KW - Gastrula

KW - Gastrulation

KW - Models, Biological

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JO - Trends in Cell Biology

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3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation

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