A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity

William R A Brown, Gianni Liti, Carlos Rosa, Steve James, Ian Roberts, Vincent Robert, Neil Jolly, Wen Tang, Peter Baumann, Carter Green, Kristina Schlegel, Jonathan Young, Fabienne Hirchaud, Spencer Leek, Geraint Thomas, Anders Blomberg, Jonas Warringer

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, π, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.

    Originele taal-2Engels
    Pagina's (van-tot)615-26
    Aantal pagina's12
    TijdschriftG3: Genes, Genomes, Genetics
    Volume1
    Nummer van het tijdschrift7
    DOI's
    StatusGepubliceerd - dec 2011

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