A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

Norman Sachs; Joep de Ligt; Oded Kopper; Ewa Gogola; Gergana Bounova; Fleur Weeber; Anjali Vanita Balgobind; Karin Wind; Ana Gracanin; Harry Begthel; Jeroen Korving; Ruben van Boxtel; Alexandra Alves Duarte; Daphne Lelieveld; Arne van Hoeck; Robert Frans Ernst; Francis Blokzijl; Isaac Johannes Nijman; Marlous Hoogstraat; Marieke van der Ven; David Anthony Egan; Vittoria Zinzalla; Jurgen Moll; Sylvia Fernandez Boj; Emile Eugene Voest; Lodewyk Wessels; Paul Joannes van Diest; Sven Rottenberg; Robert Gerhardus Jacob Vries; Edwin Cuppen; Hans Clevers

doi:10.1016/j.cell.2017.11.010

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

Norman Sachs, Joep de Ligt, Oded Kopper, Ewa Gogola, Gergana Bounova, Fleur Weeber, Anjali Vanita Balgobind, Karin Wind, Ana Gracanin, Harry Begthel, Jeroen Korving, Ruben van Boxtel, Alexandra Alves Duarte, Daphne Lelieveld, Arne van Hoeck, Robert Frans Ernst, Francis Blokzijl, Isaac Johannes Nijman, Marlous Hoogstraat, Marieke van der VenDavid Anthony Egan, Vittoria Zinzalla, Jurgen Moll, Sylvia Fernandez Boj, Emile Eugene Voest, Lodewyk Wessels, Paul Joannes van Diest, Sven Rottenberg, Robert Gerhardus Jacob Vries, Edwin Cuppen, Hans Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

1204 Citaten (Scopus)

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Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating the diversity of the disease. BC organoid morphologies typically matched the histopathology, hormone receptor status, and HER2 status of the original tumor. DNA copy number variations as well as sequence changes were consistent within tumor-organoid pairs and largely retained even after extended passaging. BC organoids furthermore populated all major gene-expression-based classification groups and allowed in vitro drug screens that were consistent with in vivo xeno-transplantations and patient response. This study describes a representative collection of well-characterized BC organoids available for cancer research and drug development, as well as a strategy to assess in vitro drug response in a personalized fashion.

Originele taal-2	Engels
Tijdschrift	Cell
DOI's	https://doi.org/10.1016/j.cell.2017.11.010
Status	Gepubliceerd - 2018

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10.1016/j.cell.2017.11.010

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Sachs, N., de Ligt, J., Kopper, O., Gogola, E., Bounova, G., Weeber, F., Balgobind, A. V., Wind, K., Gracanin, A., Begthel, H., Korving, J., van Boxtel, R., Duarte, A. A., Lelieveld, D., van Hoeck, A., Ernst, R. F., Blokzijl, F., Nijman, I. J., Hoogstraat, M., ... Clevers, H. (2018). A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. Cell. https://doi.org/10.1016/j.cell.2017.11.010

@article{1ac1b85875394a1ba4a9ba91f526c7bc,

title = "A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity",

abstract = "Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating the diversity of the disease. BC organoid morphologies typically matched the histopathology, hormone receptor status, and HER2 status of the original tumor. DNA copy number variations as well as sequence changes were consistent within tumor-organoid pairs and largely retained even after extended passaging. BC organoids furthermore populated all major gene-expression-based classification groups and allowed in vitro drug screens that were consistent with in vivo xeno-transplantations and patient response. This study describes a representative collection of well-characterized BC organoids available for cancer research and drug development, as well as a strategy to assess in vitro drug response in a personalized fashion.",

keywords = "Journal Article",

author = "Norman Sachs and {de Ligt}, Joep and Oded Kopper and Ewa Gogola and Gergana Bounova and Fleur Weeber and Balgobind, {Anjali Vanita} and Karin Wind and Ana Gracanin and Harry Begthel and Jeroen Korving and {van Boxtel}, Ruben and Duarte, {Alexandra Alves} and Daphne Lelieveld and {van Hoeck}, Arne and Ernst, {Robert Frans} and Francis Blokzijl and Nijman, {Isaac Johannes} and Marlous Hoogstraat and {van der Ven}, Marieke and Egan, {David Anthony} and Vittoria Zinzalla and Jurgen Moll and Boj, {Sylvia Fernandez} and Voest, {Emile Eugene} and Lodewyk Wessels and {van Diest}, {Paul Joannes} and Sven Rottenberg and Vries, {Robert Gerhardus Jacob} and Edwin Cuppen and Hans Clevers",

year = "2018",

doi = "10.1016/j.cell.2017.11.010",

language = "English",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

}

Sachs, N, de Ligt, J, Kopper, O, Gogola, E, Bounova, G, Weeber, F, Balgobind, AV, Wind, K, Gracanin, A, Begthel, H, Korving, J, van Boxtel, R, Duarte, AA, Lelieveld, D, van Hoeck, A, Ernst, RF, Blokzijl, F, Nijman, IJ, Hoogstraat, M, van der Ven, M, Egan, DA, Zinzalla, V, Moll, J, Boj, SF, Voest, EE, Wessels, L, van Diest, PJ, Rottenberg, S, Vries, RGJ, Cuppen, E & Clevers, H 2018, 'A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity', Cell. https://doi.org/10.1016/j.cell.2017.11.010

TY - JOUR

T1 - A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

AU - Sachs, Norman

AU - de Ligt, Joep

AU - Kopper, Oded

AU - Gogola, Ewa

AU - Bounova, Gergana

AU - Weeber, Fleur

AU - Balgobind, Anjali Vanita

AU - Wind, Karin

AU - Gracanin, Ana

AU - Begthel, Harry

AU - Korving, Jeroen

AU - van Boxtel, Ruben

AU - Duarte, Alexandra Alves

AU - Lelieveld, Daphne

AU - van Hoeck, Arne

AU - Ernst, Robert Frans

AU - Blokzijl, Francis

AU - Nijman, Isaac Johannes

AU - Hoogstraat, Marlous

AU - van der Ven, Marieke

AU - Egan, David Anthony

AU - Zinzalla, Vittoria

AU - Moll, Jurgen

AU - Boj, Sylvia Fernandez

AU - Voest, Emile Eugene

AU - Wessels, Lodewyk

AU - van Diest, Paul Joannes

AU - Rottenberg, Sven

AU - Vries, Robert Gerhardus Jacob

AU - Cuppen, Edwin

AU - Clevers, Hans

PY - 2018

Y1 - 2018

N2 - Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating the diversity of the disease. BC organoid morphologies typically matched the histopathology, hormone receptor status, and HER2 status of the original tumor. DNA copy number variations as well as sequence changes were consistent within tumor-organoid pairs and largely retained even after extended passaging. BC organoids furthermore populated all major gene-expression-based classification groups and allowed in vitro drug screens that were consistent with in vivo xeno-transplantations and patient response. This study describes a representative collection of well-characterized BC organoids available for cancer research and drug development, as well as a strategy to assess in vitro drug response in a personalized fashion.

AB - Breast cancer (BC) comprises multiple distinct subtypes that differ genetically, pathologically, and clinically. Here, we describe a robust protocol for long-term culturing of human mammary epithelial organoids. Using this protocol, >100 primary and metastatic BC organoid lines were generated, broadly recapitulating the diversity of the disease. BC organoid morphologies typically matched the histopathology, hormone receptor status, and HER2 status of the original tumor. DNA copy number variations as well as sequence changes were consistent within tumor-organoid pairs and largely retained even after extended passaging. BC organoids furthermore populated all major gene-expression-based classification groups and allowed in vitro drug screens that were consistent with in vivo xeno-transplantations and patient response. This study describes a representative collection of well-characterized BC organoids available for cancer research and drug development, as well as a strategy to assess in vitro drug response in a personalized fashion.

KW - Journal Article

U2 - 10.1016/j.cell.2017.11.010

DO - 10.1016/j.cell.2017.11.010

M3 - Article

C2 - 29224780

SN - 0092-8674

JO - Cell

JF - Cell

ER -

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

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