A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis

Caucasella Diaz-Trujillo; Pablo Chong; Ioannis Stergiopoulos; Viviane Cordovez; Mauricio Guzman; Pierre J. G. M. de Wit; H.J.G. Meijer; Gabriel Scalliet; Helge Sierotzki; Esther Lilia Peralta; Rafael E. Arango Isaza; G.H.J. Kema

doi:10.1111/mpp.12637

A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis

Caucasella Diaz-Trujillo, Pablo Chong, Ioannis Stergiopoulos, Viviane Cordovez, Mauricio Guzman, Pierre J. G. M. de Wit, H.J.G. Meijer, Gabriel Scalliet, Helge Sierotzki, Esther Lilia Peralta, Rafael E. Arango Isaza (Co-auteur), G.H.J. Kema (Co-auteur)

NIOO - Microbial Ecology (ME)

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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Summary The Dothideomycete Pseudocercospora fijiensis, previously Mycosphaerella fijiensis, is the causal agent of black Sigatoka, one of the most destructive diseases of bananas and plantains. Disease management depends on fungicide applications, with a major contribution from sterol demethylation‐inhibitors (DMIs). The continued use of DMIs places considerable selection pressure on natural P. fijiensis populations, enabling the selection of novel genotypes with reduced sensitivity. The hitherto explanatory mechanism for this reduced sensitivity was the presence of non‐synonymous point mutations in the target gene Pfcyp51, encoding the sterol 14α‐demethylase enzyme. Here, we demonstrate a second mechanism involved in DMI sensitivity of P. fijiensis. We identified a 19‐bp element in the wild‐type (wt) Pfcyp51 promoter that concatenates in strains with reduced DMI sensitivity. A polymerase chain reaction (PCR) assay identified up to six Pfcyp51 promoter repeats in four field populations of P. fijiensis in Costa Rica. We used transformation experiments to swap the wt promoter of a sensitive field isolate with a promoter from a strain with reduced DMI sensitivity that comprised multiple insertions. Comparative in vivo phenotyping showed a functional and proportional up‐regulation of Pfcyp51, which consequently decreased DMI sensitivity. Our data demonstrate that point mutations in the Pfcyp51 coding domain, as well as promoter inserts, contribute to the reduced DMI sensitivity of P. fijiensis. These results provide new insights into the importance of the appropriate use of DMIs and the need for the discovery of new molecules for black Sigatoka management.

Originele taal-2	Engels
Pagina's (van-tot)	1491-1503
Aantal pagina's	13
Tijdschrift	Molecular Plant Pathology
Volume	19
Nummer van het tijdschrift	6
DOI's	https://doi.org/10.1111/mpp.12637
Status	Gepubliceerd - jun. 2018

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10.1111/mpp.12637Licentie: CC BY

6535_Diaz-TrujilloFinal published version, 688 KBLicentie: CC BY

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Diaz-Trujillo, C., Chong, P., Stergiopoulos, I., Cordovez, V., Guzman, M., de Wit, P. J. G. M., Meijer, H. J. G., Scalliet, G., Sierotzki, H., Peralta, E. L., Arango Isaza, R. E., & Kema, G. H. J. (2018). A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis. Molecular Plant Pathology, 19(6), 1491-1503. https://doi.org/10.1111/mpp.12637

@article{5fb59dbce6c64f22a8ae3a9eeeebc408,

title = "A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis",

abstract = "Summary The Dothideomycete Pseudocercospora fijiensis, previously Mycosphaerella fijiensis, is the causal agent of black Sigatoka, one of the most destructive diseases of bananas and plantains. Disease management depends on fungicide applications, with a major contribution from sterol demethylation‐inhibitors (DMIs). The continued use of DMIs places considerable selection pressure on natural P. fijiensis populations, enabling the selection of novel genotypes with reduced sensitivity. The hitherto explanatory mechanism for this reduced sensitivity was the presence of non‐synonymous point mutations in the target gene Pfcyp51, encoding the sterol 14α‐demethylase enzyme. Here, we demonstrate a second mechanism involved in DMI sensitivity of P. fijiensis. We identified a 19‐bp element in the wild‐type (wt) Pfcyp51 promoter that concatenates in strains with reduced DMI sensitivity. A polymerase chain reaction (PCR) assay identified up to six Pfcyp51 promoter repeats in four field populations of P. fijiensis in Costa Rica. We used transformation experiments to swap the wt promoter of a sensitive field isolate with a promoter from a strain with reduced DMI sensitivity that comprised multiple insertions. Comparative in vivo phenotyping showed a functional and proportional up‐regulation of Pfcyp51, which consequently decreased DMI sensitivity. Our data demonstrate that point mutations in the Pfcyp51 coding domain, as well as promoter inserts, contribute to the reduced DMI sensitivity of P. fijiensis. These results provide new insights into the importance of the appropriate use of DMIs and the need for the discovery of new molecules for black Sigatoka management.",

keywords = "DMI, fungicide, Pfcyp51 promoter, international",

author = "Caucasella Diaz-Trujillo and Pablo Chong and Ioannis Stergiopoulos and Viviane Cordovez and Mauricio Guzman and {de Wit}, {Pierre J. G. M.} and H.J.G. Meijer and Gabriel Scalliet and Helge Sierotzki and Peralta, {Esther Lilia} and {Arango Isaza}, {Rafael E.} and G.H.J. Kema",

note = "6535, ME; Data archiving: data property of and archived at WUR",

year = "2018",

month = jun,

doi = "10.1111/mpp.12637",

language = "English",

volume = "19",

pages = "1491--1503",

journal = "Molecular Plant Pathology",

issn = "1464-6722",

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Diaz-Trujillo, C, Chong, P, Stergiopoulos, I, Cordovez, V, Guzman, M, de Wit, PJGM, Meijer, HJG, Scalliet, G, Sierotzki, H, Peralta, EL, Arango Isaza, RE & Kema, GHJ 2018, 'A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis', Molecular Plant Pathology, vol. 19, nr. 6, blz. 1491-1503. https://doi.org/10.1111/mpp.12637

A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis. / Diaz-Trujillo, Caucasella; Chong, Pablo; Stergiopoulos, Ioannis et al.
In: Molecular Plant Pathology, Vol. 19, Nr. 6, 06.2018, blz. 1491-1503.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis

AU - Diaz-Trujillo, Caucasella

AU - Chong, Pablo

AU - Stergiopoulos, Ioannis

AU - Cordovez, Viviane

AU - Guzman, Mauricio

AU - de Wit, Pierre J. G. M.

AU - Meijer, H.J.G.

AU - Scalliet, Gabriel

AU - Sierotzki, Helge

AU - Peralta, Esther Lilia

AU - Arango Isaza, Rafael E.

AU - Kema, G.H.J.

N1 - 6535, ME; Data archiving: data property of and archived at WUR

PY - 2018/6

Y1 - 2018/6

N2 - Summary The Dothideomycete Pseudocercospora fijiensis, previously Mycosphaerella fijiensis, is the causal agent of black Sigatoka, one of the most destructive diseases of bananas and plantains. Disease management depends on fungicide applications, with a major contribution from sterol demethylation‐inhibitors (DMIs). The continued use of DMIs places considerable selection pressure on natural P. fijiensis populations, enabling the selection of novel genotypes with reduced sensitivity. The hitherto explanatory mechanism for this reduced sensitivity was the presence of non‐synonymous point mutations in the target gene Pfcyp51, encoding the sterol 14α‐demethylase enzyme. Here, we demonstrate a second mechanism involved in DMI sensitivity of P. fijiensis. We identified a 19‐bp element in the wild‐type (wt) Pfcyp51 promoter that concatenates in strains with reduced DMI sensitivity. A polymerase chain reaction (PCR) assay identified up to six Pfcyp51 promoter repeats in four field populations of P. fijiensis in Costa Rica. We used transformation experiments to swap the wt promoter of a sensitive field isolate with a promoter from a strain with reduced DMI sensitivity that comprised multiple insertions. Comparative in vivo phenotyping showed a functional and proportional up‐regulation of Pfcyp51, which consequently decreased DMI sensitivity. Our data demonstrate that point mutations in the Pfcyp51 coding domain, as well as promoter inserts, contribute to the reduced DMI sensitivity of P. fijiensis. These results provide new insights into the importance of the appropriate use of DMIs and the need for the discovery of new molecules for black Sigatoka management.

AB - Summary The Dothideomycete Pseudocercospora fijiensis, previously Mycosphaerella fijiensis, is the causal agent of black Sigatoka, one of the most destructive diseases of bananas and plantains. Disease management depends on fungicide applications, with a major contribution from sterol demethylation‐inhibitors (DMIs). The continued use of DMIs places considerable selection pressure on natural P. fijiensis populations, enabling the selection of novel genotypes with reduced sensitivity. The hitherto explanatory mechanism for this reduced sensitivity was the presence of non‐synonymous point mutations in the target gene Pfcyp51, encoding the sterol 14α‐demethylase enzyme. Here, we demonstrate a second mechanism involved in DMI sensitivity of P. fijiensis. We identified a 19‐bp element in the wild‐type (wt) Pfcyp51 promoter that concatenates in strains with reduced DMI sensitivity. A polymerase chain reaction (PCR) assay identified up to six Pfcyp51 promoter repeats in four field populations of P. fijiensis in Costa Rica. We used transformation experiments to swap the wt promoter of a sensitive field isolate with a promoter from a strain with reduced DMI sensitivity that comprised multiple insertions. Comparative in vivo phenotyping showed a functional and proportional up‐regulation of Pfcyp51, which consequently decreased DMI sensitivity. Our data demonstrate that point mutations in the Pfcyp51 coding domain, as well as promoter inserts, contribute to the reduced DMI sensitivity of P. fijiensis. These results provide new insights into the importance of the appropriate use of DMIs and the need for the discovery of new molecules for black Sigatoka management.

KW - DMI, fungicide, Pfcyp51 promoter

KW - international

U2 - 10.1111/mpp.12637

DO - 10.1111/mpp.12637

M3 - Article

SN - 1464-6722

VL - 19

SP - 1491

EP - 1503

JO - Molecular Plant Pathology

JF - Molecular Plant Pathology

IS - 6

ER -

A new mechanism for reduced sensitivity to demethylation‐inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis

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