A new perspective on fungal metabolites: identification of bioactive compounds from fungi using zebrafish embryogenesis as read-out

Jelmer Hoeksma, Tim Misset, Christie Wever, Johan Kemmink, John Kruijtzer, Kees Versluis, Rob M J Liskamp, Geert Jan Boons, Albert J R Heck, Teun Boekhout, Jeroen den Hertog

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

There is a constant need for new therapeutic compounds. Fungi have proven to be an excellent, but underexplored source for biologically active compounds with therapeutic potential. Here, we combine mycology, embryology and chemistry by testing secondary metabolites from more than 10,000 species of fungi for biological activity using developing zebrafish (Danio rerio) embryos. Zebrafish development is an excellent model for high-throughput screening. Development is rapid, multiple cell types are assessed simultaneously and embryos are available in high numbers. We found that 1,526 fungal strains produced secondary metabolites with biological activity in the zebrafish bioassay. The active compounds from 39 selected fungi were purified by liquid-liquid extraction and preparative HPLC. 34 compounds were identified by a combination of chemical analyses, including LCMS, UV-Vis spectroscopy/ spectrophotometry, high resolution mass spectrometry and NMR. Our results demonstrate that fungi express a wide variety of biologically active compounds, consisting of both known therapeutic compounds as well as relatively unexplored compounds. Understanding their biological activity in zebrafish may provide insight into underlying biological processes as well as mode of action. Together, this information may provide the first step towards lead compound development for therapeutic drug development.

Originele taal-2Engels
Pagina's (van-tot)17546
TijdschriftScientific Reports
Volume9
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 26 nov 2019

Vingerafdruk Duik in de onderzoeksthema's van 'A new perspective on fungal metabolites: identification of bioactive compounds from fungi using zebrafish embryogenesis as read-out'. Samen vormen ze een unieke vingerafdruk.

  • Citeer dit

    Hoeksma, J., Misset, T., Wever, C., Kemmink, J., Kruijtzer, J., Versluis, K., Liskamp, R. M. J., Boons, G. J., Heck, A. J. R., Boekhout, T., & den Hertog, J. (2019). A new perspective on fungal metabolites: identification of bioactive compounds from fungi using zebrafish embryogenesis as read-out. Scientific Reports, 9(1), 17546. https://doi.org/10.1038/s41598-019-54127-9