A novel l-arabinose-responsive regulator discovered in the rice-blast fungus Pyricularia oryzae (Magnaporthe oryzae)

Sylvia Klaubauf; Miaomiao Zhou; Marc-Henri Lebrun; Ronald P de Vries; Evy Battaglia

doi:10.1002/1873-3468.12070

A novel l-arabinose-responsive regulator discovered in the rice-blast fungus Pyricularia oryzae (Magnaporthe oryzae)

Sylvia Klaubauf, Miaomiao Zhou, Marc-Henri Lebrun, Ronald P de Vries, Evy Battaglia

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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In this study we identified the l-arabinose-responsive regulator of Pyricularia oryzae that regulates l-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF), AraR, that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on l-arabinose. Deletion mutants of these genes were screened for their growth phenotype on l-arabinose. One mutant, named Δara1, was further analyzed. Our analysis demonstrated that Ara1 from P. oryzae is the functional analog of AraR from A. niger, while there is no significant sequence similarity between them.

Originele taal-2	Engels
Pagina's (van-tot)	550-8
Aantal pagina's	9
Tijdschrift	FEBS Letters
Volume	590
Nummer van het tijdschrift	4
DOI's	https://doi.org/10.1002/1873-3468.12070
Status	Gepubliceerd - feb. 2016

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10.1002/1873-3468.12070

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title = "A novel l-arabinose-responsive regulator discovered in the rice-blast fungus Pyricularia oryzae (Magnaporthe oryzae)",

abstract = "In this study we identified the l-arabinose-responsive regulator of Pyricularia oryzae that regulates l-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF), AraR, that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on l-arabinose. Deletion mutants of these genes were screened for their growth phenotype on l-arabinose. One mutant, named Δara1, was further analyzed. Our analysis demonstrated that Ara1 from P. oryzae is the functional analog of AraR from A. niger, while there is no significant sequence similarity between them.",

author = "Sylvia Klaubauf and Miaomiao Zhou and Marc-Henri Lebrun and {de Vries}, {Ronald P} and Evy Battaglia",

note = "{\textcopyright} 2016 Federation of European Biochemical Societies.",

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AU - de Vries, Ronald P

AU - Battaglia, Evy

PY - 2016/2

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N2 - In this study we identified the l-arabinose-responsive regulator of Pyricularia oryzae that regulates l-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF), AraR, that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on l-arabinose. Deletion mutants of these genes were screened for their growth phenotype on l-arabinose. One mutant, named Δara1, was further analyzed. Our analysis demonstrated that Ara1 from P. oryzae is the functional analog of AraR from A. niger, while there is no significant sequence similarity between them.

AB - In this study we identified the l-arabinose-responsive regulator of Pyricularia oryzae that regulates l-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF), AraR, that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on l-arabinose. Deletion mutants of these genes were screened for their growth phenotype on l-arabinose. One mutant, named Δara1, was further analyzed. Our analysis demonstrated that Ara1 from P. oryzae is the functional analog of AraR from A. niger, while there is no significant sequence similarity between them.

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A novel l-arabinose-responsive regulator discovered in the rice-blast fungus Pyricularia oryzae (Magnaporthe oryzae)

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