A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression

TY - JOUR

T1 - A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression

AU - Vrij, Erik J

AU - Scholte Op Reimer, Yvonne S

AU - Roa Fuentes, Laury

AU - Misteli Guerreiro, Isabel

AU - Holzmann, Viktoria

AU - Frias Aldeguer, Javier

AU - Sestini, Giovanni

AU - Koo, Bon-Kyoung

AU - Kind, Jop

AU - van Blitterswijk, Clemens A

AU - Rivron, Nicolas C

N1 - © 2022. Published by The Company of Biologists Ltd.

PY - 2022/10/15

Y1 - 2022/10/15

N2 - Embryogenesis is supported by dynamic loops of cellular interactions. Here, we create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn). We trigger naive mouse embryonic stem cells to form a blastocyst-stage niche of Epi-like cells and XEn-like cells (3D, hydrogel free and serum free). Once established, these two lineages autonomously progress in minimal medium to form an inner pro-amniotic-like cavity surrounded by polarized Epi-like cells covered with visceral endoderm (VE)-like cells. The progression occurs through reciprocal inductions by which the Epi supports the primitive endoderm (PrE) to produce a basal lamina that subsequently regulates Epi polarization and/or cavitation, which, in return, channels the transcriptomic progression to VE. This VE then contributes to Epi bifurcation into anterior- and posterior-like states. Similarly, boosting the formation of PrE-like cells within blastoids supports developmental progression. We argue that self-organization can arise from lineage bifurcation followed by a pendulum of induction that propagates over time.

AB - Embryogenesis is supported by dynamic loops of cellular interactions. Here, we create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn). We trigger naive mouse embryonic stem cells to form a blastocyst-stage niche of Epi-like cells and XEn-like cells (3D, hydrogel free and serum free). Once established, these two lineages autonomously progress in minimal medium to form an inner pro-amniotic-like cavity surrounded by polarized Epi-like cells covered with visceral endoderm (VE)-like cells. The progression occurs through reciprocal inductions by which the Epi supports the primitive endoderm (PrE) to produce a basal lamina that subsequently regulates Epi polarization and/or cavitation, which, in return, channels the transcriptomic progression to VE. This VE then contributes to Epi bifurcation into anterior- and posterior-like states. Similarly, boosting the formation of PrE-like cells within blastoids supports developmental progression. We argue that self-organization can arise from lineage bifurcation followed by a pendulum of induction that propagates over time.

KW - Animals

KW - Blastocyst

KW - Cell Differentiation

KW - Cell Lineage/physiology

KW - Embryo Implantation

KW - Embryo, Mammalian

KW - Endoderm

KW - Germ Layers

KW - Mice

U2 - 10.1242/dev.192310

DO - 10.1242/dev.192310

M3 - Article

C2 - 35993866

SN - 0950-1991

VL - 149

JO - Development (Cambridge)

JF - Development (Cambridge)

IS - 20

ER -