A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Barbara J Klotz, Loes A Oosterhoff, Lizette Utomo, Khoon S Lim, Queralt Vallmajo-Martin, Hans Clevers, Tim B F Woodfield, Antoine J W P Rosenberg, Jos Malda, Martin Ehrbar, Bart Spee, Debby Gawlitta

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

For creating functional tissue analogues in tissue engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment in a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed of poly(ethylene glycol) (PEG), which are spiked with peptides, have been recently developed and shown equivalence to Matrigel for stem cell differentiation. Here a clinically relevant hydrogel platform, based on PEG and gelatin, which even outperforms Matrigel when targeting 3D prevascularized bone and liver organoid tissue engineering models is presented. The hybrid hydrogel with natural and synthetic components stimulates efficient cell differentiation, superior to Matrigel models. Furthermore, the strength of this hydrogel lies in the option to covalently incorporate unmodified proteins. These results demonstrate how a hybrid hydrogel platform with intermediate biological complexity, when compared to existing biological materials and synthetic PEG-peptide approaches, can efficiently support tissue development from human primary cells.

Originele taal-2Engels
Pagina's (van-tot)e1900979
TijdschriftAdvanced healthcare materials
Volume8
Nummer van het tijdschrift19
DOI's
StatusGepubliceerd - okt 2019

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