A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Barbara J Klotz; Loes A Oosterhoff; Lizette Utomo; Khoon S Lim; Queralt Vallmajo-Martin; Hans Clevers; Tim B F Woodfield; Antoine J W P Rosenberg; Jos Malda; Martin Ehrbar; Bart Spee; Debby Gawlitta

doi:10.1002/adhm.201900979

A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Barbara J Klotz, Loes A Oosterhoff, Lizette Utomo, Khoon S Lim, Queralt Vallmajo-Martin, Hans Clevers, Tim B F Woodfield, Antoine J W P Rosenberg, Jos Malda, Martin Ehrbar, Bart Spee, Debby Gawlitta

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

78 Citaten (Scopus)

Samenvatting

For creating functional tissue analogues in tissue engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment in a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed of poly(ethylene glycol) (PEG), which are spiked with peptides, have been recently developed and shown equivalence to Matrigel for stem cell differentiation. Here a clinically relevant hydrogel platform, based on PEG and gelatin, which even outperforms Matrigel when targeting 3D prevascularized bone and liver organoid tissue engineering models is presented. The hybrid hydrogel with natural and synthetic components stimulates efficient cell differentiation, superior to Matrigel models. Furthermore, the strength of this hydrogel lies in the option to covalently incorporate unmodified proteins. These results demonstrate how a hybrid hydrogel platform with intermediate biological complexity, when compared to existing biological materials and synthetic PEG-peptide approaches, can efficiently support tissue development from human primary cells.

Originele taal-2	Engels
Pagina's (van-tot)	e1900979
Tijdschrift	Advanced healthcare materials
Volume	8
Nummer van het tijdschrift	19
DOI's	https://doi.org/10.1002/adhm.201900979
Status	Gepubliceerd - okt. 2019

Toegang tot document

10.1002/adhm.201900979

Citeer dit

@article{f38ac7ca03bc4195a172ee02f10d75a6,

title = "A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues",

abstract = "For creating functional tissue analogues in tissue engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment in a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed of poly(ethylene glycol) (PEG), which are spiked with peptides, have been recently developed and shown equivalence to Matrigel for stem cell differentiation. Here a clinically relevant hydrogel platform, based on PEG and gelatin, which even outperforms Matrigel when targeting 3D prevascularized bone and liver organoid tissue engineering models is presented. The hybrid hydrogel with natural and synthetic components stimulates efficient cell differentiation, superior to Matrigel models. Furthermore, the strength of this hydrogel lies in the option to covalently incorporate unmodified proteins. These results demonstrate how a hybrid hydrogel platform with intermediate biological complexity, when compared to existing biological materials and synthetic PEG-peptide approaches, can efficiently support tissue development from human primary cells.",

author = "Klotz, {Barbara J} and Oosterhoff, {Loes A} and Lizette Utomo and Lim, {Khoon S} and Queralt Vallmajo-Martin and Hans Clevers and Woodfield, {Tim B F} and Rosenberg, {Antoine J W P} and Jos Malda and Martin Ehrbar and Bart Spee and Debby Gawlitta",

note = "{\textcopyright} 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2019",

month = oct,

doi = "10.1002/adhm.201900979",

language = "English",

volume = "8",

pages = "e1900979",

journal = "Advanced healthcare materials",

issn = "2192-2640",

publisher = "John Wiley and Sons Ltd",

number = "19",

}

TY - JOUR

T1 - A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

AU - Klotz, Barbara J

AU - Oosterhoff, Loes A

AU - Utomo, Lizette

AU - Lim, Khoon S

AU - Vallmajo-Martin, Queralt

AU - Clevers, Hans

AU - Woodfield, Tim B F

AU - Rosenberg, Antoine J W P

AU - Malda, Jos

AU - Ehrbar, Martin

AU - Spee, Bart

AU - Gawlitta, Debby

PY - 2019/10

Y1 - 2019/10

N2 - For creating functional tissue analogues in tissue engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment in a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed of poly(ethylene glycol) (PEG), which are spiked with peptides, have been recently developed and shown equivalence to Matrigel for stem cell differentiation. Here a clinically relevant hydrogel platform, based on PEG and gelatin, which even outperforms Matrigel when targeting 3D prevascularized bone and liver organoid tissue engineering models is presented. The hybrid hydrogel with natural and synthetic components stimulates efficient cell differentiation, superior to Matrigel models. Furthermore, the strength of this hydrogel lies in the option to covalently incorporate unmodified proteins. These results demonstrate how a hybrid hydrogel platform with intermediate biological complexity, when compared to existing biological materials and synthetic PEG-peptide approaches, can efficiently support tissue development from human primary cells.

AB - For creating functional tissue analogues in tissue engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment in a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed of poly(ethylene glycol) (PEG), which are spiked with peptides, have been recently developed and shown equivalence to Matrigel for stem cell differentiation. Here a clinically relevant hydrogel platform, based on PEG and gelatin, which even outperforms Matrigel when targeting 3D prevascularized bone and liver organoid tissue engineering models is presented. The hybrid hydrogel with natural and synthetic components stimulates efficient cell differentiation, superior to Matrigel models. Furthermore, the strength of this hydrogel lies in the option to covalently incorporate unmodified proteins. These results demonstrate how a hybrid hydrogel platform with intermediate biological complexity, when compared to existing biological materials and synthetic PEG-peptide approaches, can efficiently support tissue development from human primary cells.

U2 - 10.1002/adhm.201900979

DO - 10.1002/adhm.201900979

M3 - Article

C2 - 31402634

SN - 2192-2640

VL - 8

SP - e1900979

JO - Advanced healthcare materials

JF - Advanced healthcare materials

IS - 19

ER -

A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit