A versatile genome-scale PCR-based pipeline for high-definition DNA FISH

M. Bienko; N. Crosetto; L. Teytelman; S. Klemm; S. Itzkovitz; A. van Oudenaarden

doi:10.1038/NMETH.2306

A versatile genome-scale PCR-based pipeline for high-definition DNA FISH

M. Bienko, N. Crosetto, L. Teytelman, S. Klemm, S. Itzkovitz, A. van Oudenaarden

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

49 Citaten (Scopus)

Samenvatting

We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.

Originele taal-2	Engels
Pagina's (van-tot)	122-124
Tijdschrift	Nature Methods
Volume	10
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1038/NMETH.2306
Status	Gepubliceerd - 2013

Toegang tot document

10.1038/NMETH.2306

Citeer dit

@article{526fd1f5f1b34be69da2879470dc2d6d,

title = "A versatile genome-scale PCR-based pipeline for high-definition DNA FISH",

abstract = "We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.",

author = "M. Bienko and N. Crosetto and L. Teytelman and S. Klemm and S. Itzkovitz and {van Oudenaarden}, A.",

note = "Reporting year: 2013",

year = "2013",

doi = "10.1038/NMETH.2306",

language = "English",

volume = "10",

pages = "122--124",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "2",

}

TY - JOUR

T1 - A versatile genome-scale PCR-based pipeline for high-definition DNA FISH

AU - Bienko, M.

AU - Crosetto, N.

AU - Teytelman, L.

AU - Klemm, S.

AU - Itzkovitz, S.

AU - van Oudenaarden, A.

N1 - Reporting year: 2013

PY - 2013

Y1 - 2013

N2 - We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.

AB - We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.

U2 - 10.1038/NMETH.2306

DO - 10.1038/NMETH.2306

M3 - Article

SN - 1548-7091

VL - 10

SP - 122

EP - 124

JO - Nature Methods

JF - Nature Methods

IS - 2

ER -

A versatile genome-scale PCR-based pipeline for high-definition DNA FISH

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit